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  1. +13
    -12
      chapters/1-introduction.tex
  2. +3
    -0
      chapters/2-methods.tex
  3. BIN
      images/biotin-streptavidin.pdf
  4. +380
    -0
      images_src/biotin-streptavidin.svg

+ 13
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chapters/1-introduction.tex View File

@ -134,7 +134,7 @@ di veicolare informazioni e
sostanze tra una cellula e l'altra, guidare la loro proliferazione o migrazione, mantenere la stabilità dei tessuti o avviarne la
riparazione quando necessario.
\begin{figure}
\begin{figure}[ht]
\centering
\includegraphics[width=0.5\linewidth]{images/adjunc.pdf}
\caption{Sequenza di cellule connesse da \emph{giunzioni aderenti} (sopra) e dettaglio di una giunzione aderente, con indicazione delle principali proteine coinvolte (sotto)}
@ -189,7 +189,7 @@ Lo stato attuale delle conoscenze sulla rete di interazioni che governa e
regola il funzionamento delle giunzioni aderenti è riportato in Appendice,
sotto forma di diagramma delle vie di segnalazione.
\begin{figure}
\begin{figure}[ht]
\centering
\includegraphics{images/aj.pdf}
\caption{Ruolo di \textbf{caderina} e catenine nelle \textit{giunzioni aderenti}}
@ -209,7 +209,7 @@ che modulano e regolano il loro funzionamento.
Come nel caso delle giunzioni aderenti questo emerge dall'interazione
tra un certo numero di proteine interagenti.
\begin{figure}
\begin{figure}[ht]
\centering
\includegraphics{images/tj.pdf}
\caption{Ruolo di \textbf{ZO-1} nelle \emph{giunzioni occludenti}}
@ -281,9 +281,16 @@ covalentemente molecole di \textit{streptavidina} alla loro superificie.
In questo modo è possibile successivamente ottenere il legame delle
microsfere col polimero biologico d'interesse, purché esso sia stato
preventivamente biotilinato. Si sfrutta in questo modo il legame
streptavidina-biotina, estremamente stabile e praticamente irreversibile.
streptavidina-biotina, estremamente stabile e praticamente irreversibile
(vedi figura \ref{fig:biotin-streptavidin}).
\begin{figure}[ht]
\centering
\includegraphics[width=0.5\linewidth]{images/biotin-streptavidin.pdf}
\caption{Manipolazione di una proteina target utilizzando una
microsfera intrappolata e il legame biotina-streptavidina.}
\label{fig:biotin-streptavidin}
\end{figure}
Per descrivere quantitativamente il funzionamento delle pinzette
ottiche consideriamo in generale l'effetto dell'interazione tra
@ -394,10 +401,4 @@ Il valore di $k$ per una certa trappola ottica, come vedremo, può essere
determinato attraverso un'apposita procedura di calibrazione che sfrutta
la diffusione della microsfera all'interno della trappola.
\section{Spettroscopia force-clamp}
\section{\textit{Imaging} di singola molecola}
\section{Microscopia di fluorescenza}

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chapters/2-methods.tex View File

@ -1,6 +1,9 @@
%%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%%
\chapter{Metodi}
\section{Force-clamp}
In figura \ref{fig:setup} è mostrato uno schema integrale
dell'apparato realizzato.
Le componenti principali sono descritte nella sezione \ref{sec:setup}.


BIN
images/biotin-streptavidin.pdf View File


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