Browse Source

Update on Overleaf.

master
lorenzo.zolfanelli93 4 years ago
committed by overleaf
parent
commit
2d5ab5fba8
4 changed files with 396 additions and 12 deletions
  1. +13
    -12
      chapters/1-introduction.tex
  2. +3
    -0
      chapters/2-methods.tex
  3. BIN
      images/biotin-streptavidin.pdf
  4. +380
    -0
      images_src/biotin-streptavidin.svg

+ 13
- 12
chapters/1-introduction.tex View File

@ -134,7 +134,7 @@ di veicolare informazioni e
sostanze tra una cellula e l'altra, guidare la loro proliferazione o migrazione, mantenere la stabilità dei tessuti o avviarne la sostanze tra una cellula e l'altra, guidare la loro proliferazione o migrazione, mantenere la stabilità dei tessuti o avviarne la
riparazione quando necessario. riparazione quando necessario.
\begin{figure}
\begin{figure}[ht]
\centering \centering
\includegraphics[width=0.5\linewidth]{images/adjunc.pdf} \includegraphics[width=0.5\linewidth]{images/adjunc.pdf}
\caption{Sequenza di cellule connesse da \emph{giunzioni aderenti} (sopra) e dettaglio di una giunzione aderente, con indicazione delle principali proteine coinvolte (sotto)} \caption{Sequenza di cellule connesse da \emph{giunzioni aderenti} (sopra) e dettaglio di una giunzione aderente, con indicazione delle principali proteine coinvolte (sotto)}
@ -189,7 +189,7 @@ Lo stato attuale delle conoscenze sulla rete di interazioni che governa e
regola il funzionamento delle giunzioni aderenti è riportato in Appendice, regola il funzionamento delle giunzioni aderenti è riportato in Appendice,
sotto forma di diagramma delle vie di segnalazione. sotto forma di diagramma delle vie di segnalazione.
\begin{figure}
\begin{figure}[ht]
\centering \centering
\includegraphics{images/aj.pdf} \includegraphics{images/aj.pdf}
\caption{Ruolo di \textbf{caderina} e catenine nelle \textit{giunzioni aderenti}} \caption{Ruolo di \textbf{caderina} e catenine nelle \textit{giunzioni aderenti}}
@ -209,7 +209,7 @@ che modulano e regolano il loro funzionamento.
Come nel caso delle giunzioni aderenti questo emerge dall'interazione Come nel caso delle giunzioni aderenti questo emerge dall'interazione
tra un certo numero di proteine interagenti. tra un certo numero di proteine interagenti.
\begin{figure}
\begin{figure}[ht]
\centering \centering
\includegraphics{images/tj.pdf} \includegraphics{images/tj.pdf}
\caption{Ruolo di \textbf{ZO-1} nelle \emph{giunzioni occludenti}} \caption{Ruolo di \textbf{ZO-1} nelle \emph{giunzioni occludenti}}
@ -281,9 +281,16 @@ covalentemente molecole di \textit{streptavidina} alla loro superificie.
In questo modo è possibile successivamente ottenere il legame delle In questo modo è possibile successivamente ottenere il legame delle
microsfere col polimero biologico d'interesse, purché esso sia stato microsfere col polimero biologico d'interesse, purché esso sia stato
preventivamente biotilinato. Si sfrutta in questo modo il legame preventivamente biotilinato. Si sfrutta in questo modo il legame
streptavidina-biotina, estremamente stabile e praticamente irreversibile.
streptavidina-biotina, estremamente stabile e praticamente irreversibile
(vedi figura \ref{fig:biotin-streptavidin}).
\begin{figure}[ht]
\centering
\includegraphics[width=0.5\linewidth]{images/biotin-streptavidin.pdf}
\caption{Manipolazione di una proteina target utilizzando una
microsfera intrappolata e il legame biotina-streptavidina.}
\label{fig:biotin-streptavidin}
\end{figure}
Per descrivere quantitativamente il funzionamento delle pinzette Per descrivere quantitativamente il funzionamento delle pinzette
ottiche consideriamo in generale l'effetto dell'interazione tra ottiche consideriamo in generale l'effetto dell'interazione tra
@ -394,10 +401,4 @@ Il valore di $k$ per una certa trappola ottica, come vedremo, può essere
determinato attraverso un'apposita procedura di calibrazione che sfrutta determinato attraverso un'apposita procedura di calibrazione che sfrutta
la diffusione della microsfera all'interno della trappola. la diffusione della microsfera all'interno della trappola.
\section{Spettroscopia force-clamp}
\section{\textit{Imaging} di singola molecola}
\section{Microscopia di fluorescenza}

+ 3
- 0
chapters/2-methods.tex View File

@ -1,6 +1,9 @@
%%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%%
\chapter{Metodi} \chapter{Metodi}
\section{Force-clamp}
In figura \ref{fig:setup} è mostrato uno schema integrale In figura \ref{fig:setup} è mostrato uno schema integrale
dell'apparato realizzato. dell'apparato realizzato.
Le componenti principali sono descritte nella sezione \ref{sec:setup}. Le componenti principali sono descritte nella sezione \ref{sec:setup}.


BIN
images/biotin-streptavidin.pdf View File


+ 380
- 0
images_src/biotin-streptavidin.svg View File

@ -0,0 +1,380 @@
<?xml version="1.0" encoding="UTF-8" standalone="no"?>
<svg
xmlns:osb="http://www.openswatchbook.org/uri/2009/osb"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:cc="http://creativecommons.org/ns#"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:svg="http://www.w3.org/2000/svg"
xmlns="http://www.w3.org/2000/svg"
xmlns:xlink="http://www.w3.org/1999/xlink"
xmlns:sodipodi="http://sodipodi.sourceforge.net/DTD/sodipodi-0.dtd"
xmlns:inkscape="http://www.inkscape.org/namespaces/inkscape"
sodipodi:docname="biotin-streptavidin.svg"
inkscape:version="1.0 (4035a4fb49, 2020-05-01)"
id="svg3131"
version="1.1"
viewBox="0 0 137.64877 100.17954"
height="100.17954mm"
width="137.64877mm">
<defs
id="defs3125">
<linearGradient
id="linearGradient5876"
inkscape:collect="always">
<stop
id="stop5872"
offset="0"
style="stop-color:#ffffff;stop-opacity:1" />
<stop
style="stop-color:#ff0000;stop-opacity:1"
offset="0.5"
id="stop5880" />
<stop
id="stop5874"
offset="1"
style="stop-color:#ffffff;stop-opacity:1" />
</linearGradient>
<inkscape:path-effect
effect="bspline"
id="path-effect5834"
is_visible="true"
lpeversion="1"
weight="33.333333"
steps="2"
helper_size="0"
apply_no_weight="true"
apply_with_weight="true"
only_selected="false" />
<inkscape:path-effect
effect="skeletal"
id="path-effect5828"
is_visible="true"
lpeversion="1"
pattern="M 44.905078,73.0999 A 13.857033,6.9124809 0 0 1 31.048048,80.012382 13.857033,6.9124809 0 0 1 17.191018,73.0999 13.857033,6.9124809 0 0 1 31.048048,66.187419 13.857033,6.9124809 0 0 1 44.905078,73.0999 Z"
copytype="repeated_stretched"
prop_scale="1"
scale_y_rel="false"
spacing="0"
normal_offset="0"
tang_offset="0"
prop_units="false"
vertical_pattern="false"
hide_knot="false"
fuse_tolerance="0" />
<inkscape:path-effect
effect="bspline"
id="path-effect5826"
is_visible="true"
lpeversion="1"
weight="33.333333"
steps="2"
helper_size="0"
apply_no_weight="true"
apply_with_weight="true"
only_selected="false" />
<inkscape:path-effect
only_selected="false"
apply_with_weight="true"
apply_no_weight="true"
helper_size="0"
steps="2"
weight="33.333333"
lpeversion="1"
is_visible="true"
id="path-effect5822"
effect="bspline" />
<inkscape:path-effect
fuse_tolerance="0"
hide_knot="false"
vertical_pattern="false"
prop_units="false"
tang_offset="0"
normal_offset="0"
spacing="0"
scale_y_rel="false"
prop_scale="1"
copytype="repeated_stretched"
pattern="m -4.963417,-35.315834 a 13.857033,6.9124809 0 0 1 -13.85703,6.912482 13.857033,6.9124809 0 0 1 -13.85703,-6.912482 13.857033,6.9124809 0 0 1 13.85703,-6.912481 13.857033,6.9124809 0 0 1 13.85703,6.912481 z"
lpeversion="1"
is_visible="true"
id="path-effect5786"
effect="skeletal" />
<inkscape:path-effect
only_selected="false"
apply_with_weight="true"
apply_no_weight="true"
helper_size="0"
steps="2"
weight="33.333333"
lpeversion="1"
is_visible="true"
id="path-effect5773"
effect="bspline" />
<linearGradient
inkscape:collect="always"
id="linearGradient5751">
<stop
style="stop-color:#00ffff;stop-opacity:1"
offset="0"
id="stop5755" />
<stop
style="stop-color:#000080;stop-opacity:1"
offset="1"
id="stop5757" />
</linearGradient>
<linearGradient
id="linearGradient5714"
osb:paint="gradient">
<stop
style="stop-color:#ff6600;stop-opacity:1;"
offset="0"
id="stop5726" />
<stop
style="stop-color:#ff6600;stop-opacity:0;"
offset="1"
id="stop5728" />
</linearGradient>
<inkscape:path-effect
pattern-nodetypes="cccccccc"
fuse_tolerance="0"
hide_knot="false"
vertical_pattern="true"
prop_units="false"
tang_offset="0"
normal_offset="0"
spacing="20"
scale_y_rel="true"
prop_scale="-1.05681"
copytype="repeated"
pattern="M -33.937917,22.500266 H -20.37282 V 16.33374 h 9.97882 l -4.248964,6.166526 4.248964,6.206076 h -9.97882 v -6.206076"
lpeversion="1"
is_visible="true"
id="path-effect3747"
effect="skeletal" />
<inkscape:path-effect
fuse_tolerance="0"
hide_knot="false"
vertical_pattern="false"
prop_units="false"
tang_offset="0"
normal_offset="0"
spacing="0"
scale_y_rel="false"
prop_scale="1"
copytype="repeated_stretched"
pattern="M 0.0625,0.06249583 15.908482,8.5291628 31.754464,0.06249583 V 16.723957 H 0.0625 Z"
lpeversion="1"
is_visible="true"
id="path-effect3732"
effect="skeletal" />
<inkscape:path-effect
fuse_tolerance="0"
hide_knot="false"
vertical_pattern="false"
prop_units="false"
tang_offset="0"
normal_offset="0"
spacing="0"
scale_y_rel="false"
prop_scale="1"
copytype="single_stretched"
pattern="M 0,0 H 1"
lpeversion="1"
is_visible="true"
id="path-effect3716"
effect="skeletal" />
<radialGradient
gradientUnits="userSpaceOnUse"
r="54.838627"
fy="104.1151"
fx="101.52227"
cy="104.1151"
cx="101.52227"
id="radialGradient5753"
xlink:href="#linearGradient5751"
inkscape:collect="always" />
<linearGradient
gradientTransform="translate(-193.56377,-193.06854)"
spreadMethod="pad"
gradientUnits="userSpaceOnUse"
y2="415.06912"
x2="239.11081"
y1="415.06912"
x1="99.085411"
id="linearGradient5878"
xlink:href="#linearGradient5876"
inkscape:collect="always" />
</defs>
<sodipodi:namedview
inkscape:window-maximized="1"
inkscape:window-y="0"
inkscape:window-x="0"
inkscape:window-height="1007"
inkscape:window-width="1920"
fit-margin-bottom="5"
fit-margin-right="5"
fit-margin-left="5"
fit-margin-top="5"
showgrid="false"
inkscape:document-rotation="0"
inkscape:current-layer="g3775"
inkscape:document-units="mm"
inkscape:cy="537.6386"
inkscape:cx="333.76935"
inkscape:zoom="0.70911897"
inkscape:pageshadow="2"
inkscape:pageopacity="0.0"
borderopacity="1.0"
bordercolor="#666666"
pagecolor="#ffffff"
id="base" />
<metadata
id="metadata3128">
<rdf:RDF>
<cc:Work
rdf:about="">
<dc:format>image/svg+xml</dc:format>
<dc:type
rdf:resource="http://purl.org/dc/dcmitype/StillImage" />
<dc:title></dc:title>
</cc:Work>
</rdf:RDF>
</metadata>
<g
transform="translate(-16.690192,-6.2497871)"
style="display:inline"
id="layer1"
inkscape:groupmode="layer"
inkscape:label="Livello 1">
<g
style="display:inline"
transform="matrix(0.36230099,0,0,0.36230099,13.831814,15.540954)"
id="g3775">
<g
id="g5844">
<path
id="rect5760"
style="fill:#ff0000;fill-opacity:0.5;fill-rule:evenodd;stroke:#000000;stroke-width:0.442612;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill"
d="m 169.78905,64.418716 21.89091,-10.575259 5.76719,12.029892 -21.89094,10.575258 -6.25407,-4.386696 z"
sodipodi:nodetypes="cccccc" />
<path
style="fill:#008080;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1"
d="m 216.05681,35.231505 c 0.93584,0.964949 1.26516,2.496742 1.13616,4.315748 -0.12792,1.803742 -0.71621,3.985737 -1.74328,6.203074 0,1e-6 0,1e-6 0,1e-6 -0.68928,1.57259 -1.55818,3.205642 -2.55551,4.781082 -1.951,3.081895 -4.35228,5.877055 -6.82367,7.630164 -2.47173,1.753357 -4.74828,2.26654 -6.35838,1.373199 -1.59414,-0.884488 -2.40519,-3.093854 -2.1822,-6.144889 0.22328,-3.055157 1.47931,-6.76884 3.69796,-10.259523 1.13717,-1.789164 2.47532,-3.435539 3.93652,-4.827107 0,0 0,-1e-6 0,-1e-6 2.07341,-2.044211 4.35576,-3.394674 6.38434,-3.904184 2.02239,-0.507954 3.56967,-0.135137 4.50806,0.832436 z m 27.17964,-6.20625 c -0.51041,1.84081 -2.59989,3.160384 -5.283,4.062958 -2.43718,0.884943 -5.2875,1.473981 -7.90349,1.972342 -1.69485,0.322878 -2.54096,0.476381 -4.23581,0.79926 0,0 0,0 0,0 -3.91786,0.530664 -7.76131,1.429817 -9.75734,-0.62831 -1.73419,-1.788126 -1.4383,-6.503676 4.20754,-10.706347 0,0 0,-1e-6 0,-1e-6 2.04983,-1.411643 4.47535,-2.448501 7.05818,-2.986194 4.71045,-0.980622 9.05968,-0.14065 12.03376,1.529481 3.23184,1.775446 4.3841,4.139346 3.88016,5.956811 z m 17.44646,20.705862 c -2.7227,0.09924 -4.7055,-2.836333 -7.07543,-5.50685 0,-10e-7 0,-10e-7 0,-10e-7 -0.99064,-1.165752 -1.87529,-2.143498 -2.97611,-3.356518 -0.69994,-0.77128 -1.33098,-1.466315 -2.03734,-2.257581 0,0 0,-10e-7 0,-10e-7 -3.03078,-3.261288 -6.16073,-6.688344 -5.35758,-9.584911 0.38432,-1.386057 1.72726,-2.550323 4.06032,-2.943531 2.3243,-0.391732 5.48555,0.04097 8.79102,1.749399 0,0 0,0 0,0 1.7683,0.938326 3.4255,2.226298 4.84419,3.823341 2.21996,2.499039 3.62279,5.491799 4.13537,8.451989 0,0 0,10e-7 0,10e-7 0.88674,6.412455 -2.0884,9.540975 -4.38444,9.624663 z m 0.54739,28.960293 c -1.34451,0.810168 -3.48306,0.467083 -5.43231,-1.789085 -2.05419,-2.377632 -3.36562,-6.161058 -3.25088,-9.969963 0,0 0,-10e-7 0,-10e-7 -0.0309,-1.096367 0.0102,-2.183344 0.11221,-3.242985 0.40217,-4.17715 1.68993,-7.683204 3.15738,-10.098762 1.36036,-2.288435 3.10915,-3.795454 4.86621,-3.859497 1.79319,-0.06536 3.63592,1.409431 4.73853,4.435107 1.1958,3.173463 1.36268,7.139357 0.99514,10.863151 -0.0904,0.915515 -0.21081,1.804414 -0.3485,2.649284 0,10e-7 0,10e-7 0,10e-7 -0.24359,2.82212 -1.25058,5.215447 -1.97359,6.827057 -0.72809,1.622949 -1.58915,3.417387 -2.86419,4.185693 z m 26.32358,1.985484 c 0.6317,0.939232 0.77236,2.224788 0.20281,3.749909 -0.64544,1.669905 -2.1881,3.58083 -4.69958,5.088299 -2.52989,1.518525 -5.80648,2.471622 -9.41953,2.404439 -1.02685,-0.01909 -2.05517,-0.120612 -3.07046,-0.304335 0,0 0,-10e-7 0,-10e-7 -4.13774,-0.665488 -7.43051,-2.993202 -9.2541,-5.619482 -1.82551,-2.629035 -1.8735,-4.98088 -1.10965,-6.277845 0,0 0,-10e-7 0,-10e-7 0.26914,-0.449009 0.62243,-0.782726 1.02693,-1.026467 0.40589,-0.244582 0.86201,-0.398557 1.35402,-0.500358 0,0 0,0 0,0 1.18965,-0.242028 3.26281,-0.176909 4.65305,-0.188801 1.61942,-0.01385 3.45533,-0.08119 5.23339,0.08926 0,-10e-7 0,0 0,0 0.48197,0.02693 0.9646,0.04591 1.46209,0.05951 3.22522,0.08819 7.56783,-0.09764 10.46475,0.650461 1.40478,0.344635 2.5167,0.924481 3.15628,1.875406 z m 19.65855,-21.624272 c 2.53977,1.892581 2.14243,7.535086 -1.81035,13.478432 0,0 0,10e-7 0,10e-7 -0.56575,0.879918 -1.20297,1.749453 -1.90909,2.590756 -2.7068,3.224994 -6.12292,5.658738 -9.37651,6.671684 -3.22661,1.004546 -5.54627,0.394495 -6.5626,-1.116601 -1.0124,-1.505248 -0.739,-3.789457 0.24412,-6.302189 0.96979,-2.478686 2.75447,-5.484763 5.09931,-8.225975 0.63538,-0.742786 1.31072,-1.464964 2.02093,-2.152409 0,0 0,-1e-6 0,-1e-6 4.5997,-4.551772 9.60325,-6.948929 12.29419,-4.943698 z m 20.41224,-20.82081 c 0.75782,1.11773 0.79232,2.651914 0.2085,4.441263 -0.59013,1.808692 -1.73095,3.672519 -3.03925,5.56143 0,0 0,1e-6 0,1e-6 -0.96432,1.345789 -2.10529,2.74871 -3.338,4.080665 -1.47529,1.594064 -3.10298,3.109588 -4.7842,4.358558 0,0 0,0 0,0 -3.79996,2.858995 -7.38521,3.924456 -9.45929,2.378893 -2.00856,-1.496739 -2.20699,-5.313169 -0.10201,-9.920099 0,0 0,-10e-7 0,-10e-7 0.96536,-2.109671 2.38968,-4.265256 4.2188,-6.227266 1.53848,-1.650244 3.28748,-3.080971 5.12882,-4.184966 0,0 0,0 0,0 5.31112,-3.162201 9.72422,-2.615939 11.16663,-0.488478 z"
id="path5771"
inkscape:path-effect="#path-effect5773;#path-effect5786"
inkscape:original-d="m 199.71213,59.534773 c 6.82663,-12.301828 13.65253,-24.604394 20.47769,-36.907683 12.52477,2.828523 25.04829,5.656202 37.57133,8.483207 1.68819,7.046865 3.37558,14.138979 5.06227,21.207366 -2.72063,10.190002 -5.44187,20.378864 -8.1639,30.5672 10.74736,2.328313 21.49354,4.655807 32.23922,6.982615 9.10322,-14.178765 18.20534,-28.357691 27.30692,-42.537632 4.47381,-3.032001 8.94671,-6.064609 13.41897,-9.098005" />
<path
style="fill:none;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1"
d="m 194.0306,58.981187 c 1.49688,-0.326223 3.01287,-0.656613 4.51849,-0.98474"
id="path5820"
inkscape:path-effect="#path-effect5822"
inkscape:original-d="m 194.0306,58.981187 c 1.50689,-0.280291 3.01306,-0.65576 4.51849,-0.98474" />
</g>
<g
transform="translate(-4.4509771,-17.64298)"
id="g5839">
<path
inkscape:original-d="m 249.58062,167.95051 c 6.82663,-12.30182 13.65253,-24.60439 20.47769,-36.90768 12.52477,2.82852 25.04829,5.6562 37.57133,8.48321 1.68819,7.04686 3.37558,14.13898 5.06227,21.20736 -2.72063,10.19001 -5.44187,20.37887 -8.1639,30.5672 10.74736,2.32832 21.49354,4.65581 32.23922,6.98262 9.10322,-14.17877 18.20534,-28.35769 27.30692,-42.53763 4.47381,-3.032 8.94671,-6.06461 13.41897,-9.09801"
inkscape:path-effect="#path-effect5826;#path-effect5828"
id="path5824"
d="m 265.9253,143.64724 c 0.93584,0.96495 1.26516,2.49675 1.13616,4.31575 -0.12792,1.80375 -0.71621,3.98574 -1.74328,6.20308 0,0 0,0 0,0 -0.68928,1.57259 -1.55818,3.20564 -2.55551,4.78108 -1.951,3.08189 -4.35228,5.87705 -6.82367,7.63016 -2.47173,1.75336 -4.74828,2.26654 -6.35838,1.3732 -1.59414,-0.88449 -2.40519,-3.09385 -2.1822,-6.14489 0.22328,-3.05516 1.47931,-6.76884 3.69796,-10.25952 1.13717,-1.78916 2.47532,-3.43554 3.93652,-4.82711 0,0 0,0 0,0 2.0734,-2.04421 4.35576,-3.39467 6.38434,-3.90418 2.02239,-0.50796 3.56967,-0.13514 4.50806,0.83243 z m 27.17964,-6.20624 c -0.51041,1.84081 -2.59989,3.16038 -5.283,4.06296 -2.43718,0.88494 -5.2875,1.47397 -7.90349,1.97233 -1.69485,0.32288 -2.54096,0.47639 -4.23581,0.79926 0,0 0,1e-5 0,1e-5 -3.91786,0.53066 -7.76131,1.42981 -9.75734,-0.62832 -1.73418,-1.78812 -1.4383,-6.50367 4.20754,-10.70634 0,0 0,0 0,0 2.04983,-1.41165 4.47535,-2.4485 7.05818,-2.9862 4.71046,-0.98062 9.05968,-0.14065 12.03376,1.52948 3.23184,1.77545 4.3841,4.13935 3.88016,5.95682 z m 17.44646,20.70586 c -2.7227,0.0992 -4.7055,-2.83633 -7.07543,-5.50685 0,0 0,0 0,0 -0.99064,-1.16575 -1.87529,-2.1435 -2.97611,-3.35652 -0.69994,-0.77128 -1.33098,-1.46632 -2.03734,-2.25758 0,0 0,0 0,0 -3.03078,-3.26129 -6.16073,-6.68835 -5.35758,-9.58491 0.38432,-1.38606 1.72726,-2.55032 4.06032,-2.94353 2.3243,-0.39174 5.48555,0.041 8.79101,1.74939 0,0 10e-6,0 10e-6,0 1.7683,0.93833 3.4255,2.2263 4.84419,3.82335 2.21996,2.49904 3.62279,5.4918 4.13537,8.45198 0,0 0,0 0,1e-5 0.88675,6.41245 -2.0884,9.54097 -4.38444,9.62466 z m 0.54739,28.96029 c -1.34451,0.81017 -3.48306,0.46709 -5.43231,-1.78908 -2.05419,-2.37763 -3.36562,-6.16106 -3.25088,-9.96996 0,0 0,-1e-5 0,-1e-5 -0.0309,-1.09636 0.0102,-2.18334 0.11221,-3.24298 0.40217,-4.17715 1.68993,-7.6832 3.15738,-10.09876 1.36036,-2.28844 3.10915,-3.79546 4.86621,-3.8595 1.79319,-0.0654 3.63592,1.40943 4.73853,4.43511 1.1958,3.17346 1.36268,7.13936 0.99514,10.86315 -0.0904,0.91551 -0.21081,1.80441 -0.3485,2.64928 0,0 0,0 0,0 -0.2436,2.82212 -1.25058,5.21545 -1.97359,6.82706 -0.72809,1.62295 -1.58915,3.41739 -2.86419,4.18569 z m 26.32358,1.98548 c 0.63171,0.93923 0.77236,2.22479 0.20281,3.74991 -0.64544,1.66991 -2.1881,3.58083 -4.69957,5.0883 -2.5299,1.51853 -5.80649,2.47162 -9.41954,2.40444 -1.02684,-0.0191 -2.05517,-0.12061 -3.07046,-0.30433 0,0 0,-1e-5 0,-1e-5 -4.13774,-0.66548 -7.43051,-2.9932 -9.25411,-5.61948 -1.8255,-2.62904 -1.87348,-4.98088 -1.10964,-6.27784 0,0 0,0 0,0 0.26914,-0.44901 0.62243,-0.78273 1.02693,-1.02647 0.40589,-0.24458 0.862,-0.39855 1.35401,-0.50035 0,0 0,0 0,0 1.18966,-0.24203 3.26281,-0.17692 4.65305,-0.18881 1.61943,-0.0138 3.45534,-0.0812 5.2334,0.0893 0,0 0,0 0,0 0.48197,0.0269 0.9646,0.0459 1.46209,0.0595 3.22522,0.0882 7.56783,-0.0976 10.46475,0.65046 1.40478,0.34463 2.51671,0.92448 3.15628,1.8754 z m 19.65855,-21.62427 c 2.53977,1.89258 2.14243,7.53509 -1.81035,13.47844 0,0 0,0 0,0 -0.56575,0.87991 -1.20297,1.74945 -1.90909,2.59075 -2.70679,3.22499 -6.12291,5.65874 -9.37651,6.67168 -3.22661,1.00455 -5.54627,0.3945 -6.5626,-1.1166 -1.0124,-1.50525 -0.739,-3.78945 0.24412,-6.30219 0.9698,-2.47868 2.75447,-5.48476 5.09931,-8.22597 0.63538,-0.74279 1.31072,-1.46496 2.02093,-2.15241 0,0 0,0 0,0 4.5997,-4.55177 9.60325,-6.94893 12.29419,-4.9437 z m 20.41224,-20.82081 c 0.75782,1.11773 0.79232,2.65191 0.2085,4.44126 -0.59012,1.8087 -1.73095,3.67253 -3.03925,5.56144 0,0 0,0 0,0 -0.96432,1.34579 -2.10529,2.74871 -3.338,4.08066 -1.47529,1.59406 -3.10298,3.10959 -4.7842,4.35856 0,0 0,0 0,0 -3.79996,2.85899 -7.38521,3.92445 -9.45929,2.37889 -2.00856,-1.49674 -2.20698,-5.31317 -0.10201,-9.92009 0,0 0,0 0,0 0.96536,-2.10968 2.38968,-4.26526 4.2188,-6.22728 1.53848,-1.65024 3.28748,-3.08096 5.12881,-4.18496 0,0 0,0 0,0 5.31113,-3.1622 9.72423,-2.61594 11.16664,-0.48848 z"
style="fill:#008080;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1" />
<path
sodipodi:nodetypes="cccccc"
d="m 217.96439,157.53731 24.30596,-0.51778 0.24424,13.33863 -24.306,0.51777 -3.86443,-6.58959 z"
style="fill:#ff0000;fill-opacity:0.5;fill-rule:evenodd;stroke:#000000;stroke-width:0.442612;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill"
id="path5830" />
<path
inkscape:original-d="m 242.27242,162.66946 c 1.48703,0.37152 3.01297,0.65616 4.51892,0.98279"
inkscape:path-effect="#path-effect5834"
id="path5832"
d="m 242.27242,162.66946 c 1.49702,0.32558 3.01315,0.65531 4.51892,0.98279"
style="fill:none;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1" />
</g>
<text
id="text5848"
y="4.0724788"
x="285.29364"
style="font-size:9.73712px;line-height:1.25;font-family:'Noto Sans';-inkscape-font-specification:'Noto Sans';letter-spacing:0px;word-spacing:0px;stroke-width:0.730286"
xml:space="preserve"><tspan
style="stroke-width:0.730286"
y="4.0724788"
x="285.29364"
id="tspan5846"
sodipodi:role="line">proteina target</tspan><tspan
id="tspan5850"
style="stroke-width:0.730286"
y="16.243877"
x="285.29364"
sodipodi:role="line" /></text>
<path
id="path5852"
d="M 281.01479,4.6686742 H 254.73431 L 248.58212,14.882551"
style="fill:none;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1" />
<text
id="text5856"
y="101.28609"
x="201.03288"
style="font-size:9.73712px;line-height:1.25;font-family:'Noto Sans';-inkscape-font-specification:'Noto Sans';letter-spacing:0px;word-spacing:0px;stroke-width:0.730286"
xml:space="preserve"><tspan
style="stroke-width:0.730286"
y="101.28609"
x="201.03288"
id="tspan5854"
sodipodi:role="line">streptavidina</tspan></text>
<path
id="path5858"
d="M 46.557759,-6.4265921 64.971701,-6.4382037 76.264997,24.082687"
style="fill:none;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1" />
<text
id="text5862"
y="194.32951"
x="163.72633"
style="font-size:9.73712px;line-height:1.25;font-family:'Noto Sans';-inkscape-font-specification:'Noto Sans';letter-spacing:0px;word-spacing:0px;stroke-width:0.730286"
xml:space="preserve"><tspan
style="stroke-width:0.730286"
y="194.32951"
x="163.72633"
id="tspan5860"
sodipodi:role="line">biotina</tspan></text>
<path
id="path5864"
d="m 198.48498,192.75886 h 20.72625 l 7.11506,-33.05849"
style="fill:none;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1" />
<g
style="display:inline"
inkscape:label="Layer 1"
id="layer2"
inkscape:groupmode="layer" />
<g
transform="matrix(1.000154,0,0,0.76922022,125.19523,-58.157631)"
id="g5870">
<path
id="rect5866"
style="fill:url(#linearGradient5878);fill-opacity:1;fill-rule:evenodd;stroke:#000000;stroke-width:0.345017;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill"
d="M -103.06929,60.380789 H 54.883247 c 0,0 -52.6508301,104.959901 -52.6508301,161.619781 0,56.65987 52.6508301,161.61978 52.6508301,161.61978 H -103.06929 c 0,0 52.650877,-104.95991 52.650877,-161.61978 0,-56.65988 -52.650877,-161.619781 -52.650877,-161.619781 z"
sodipodi:nodetypes="ccaccac" />
</g>
<circle
style="fill:url(#radialGradient5753);fill-opacity:1;fill-rule:evenodd;stroke:#000000;stroke-width:0.101412;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill"
id="path3739"
cx="101.52227"
cy="104.1151"
r="54.787922" />
<path
id="circle3745"
style="fill:#ff9955;fill-opacity:1;stroke:#000000;stroke-width:0.125;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill"
inkscape:path-effect="#path-effect3747"
sodipodi:type="arc"
sodipodi:cx="101.31975"
sodipodi:cy="104.00684"
sodipodi:rx="67.531387"
sodipodi:ry="67.531387"
d="m 156.25479,108.8538 14.14578,1.47677 c 0.22186,-2.09159 0.33895,-4.2014 0.34557,-6.32373 h 10.54572 c -1.54024,2.49419 -3.18501,4.80778 -4.91616,6.94752 1.22112,2.46283 2.30632,5.05256 3.2488,7.75077 l -10.20636,-2.12068 c 0.42973,-2.06231 0.75914,-4.14887 0.98243,-6.25388 m -23.17882,22.68458 11.84568,7.92655 c 1.16503,-1.74342 2.25518,-3.53495 3.26474,-5.3689 l 9.16043,5.05133 c -2.44881,1.38661 -4.92872,2.60627 -7.42159,3.66572 -0.0273,2.72677 -0.20411,5.50634 -0.53715,8.32031 l -8.20567,-6.55662 c 1.31839,-1.65135 2.56674,-3.35723 3.73924,-5.11184 m -31.0725,10.46887 7.35672,12.18747 c 1.79434,-1.08141 3.5437,-2.24082 5.24236,-3.47253 l 6.16495,8.49248 c -2.81499,0.19373 -5.58766,0.23415 -8.29986,0.128 -1.18845,2.4463 -2.5399,4.87131 -4.06113,7.25664 l -4.5948,-9.38236 c 1.8912,-0.92454 3.74262,-1.93388 5.54848,-3.02223 m -31.83449,-4.50081 0.67889,14.25952 c 2.10982,-0.10555 4.20453,-0.31899 6.27845,-0.63462 l 1.55929,10.32345 c -2.63879,-1.08447 -5.16183,-2.3086 -7.55098,-3.66566 -1.01837,0.72991 -2.07252,1.44049 -3.16306,2.13006 -1.332026,0.80371 -2.706108,1.56655 -4.119189,2.28736 l 0.608373,-10.47561 c 1.165794,0.0703 2.336386,0.10784 3.510816,0.11151 0.96151,-0.003 1.92046,-0.0287 2.8763,-0.0765 m -25.563657,-19.71687 -6.36043,12.70139 c 1.871205,0.93538 3.782243,1.78761 5.72742,2.55099 l -3.814358,9.70501 c -1.70171,-2.25161 -3.232691,-4.55465 -4.599689,-6.89097 -2.7082,0.30948 -5.48971,0.47325 -8.326129,0.48452 l 5.489977,-8.92059 c 1.796711,1.10404 3.639572,2.12965 5.522779,3.07104 M 58.146329,136.73982 46.919142,145.5581 c 1.296058,1.65157 2.66184,3.24988 4.091653,4.78922 l -7.701181,7.15521 c -0.532439,-2.76792 -0.907791,-5.51384 -1.132802,-8.21962 -2.578415,-0.87581 -5.156053,-1.91087 -7.71451,-3.11197 l 8.761399,-5.75539 c 1.157322,1.76426 2.391071,3.48038 3.695441,5.14255 m 0.08179,-32.33738 -13.90508,2.74629 c 0.407063,2.05391 0.913743,4.08445 1.514345,6.08592 l -9.977823,2.99522 c 0.722141,-2.74185 1.587728,-5.38826 2.590014,-7.92112 -1.904234,-1.97378 -3.733601,-4.12034 -5.469698,-6.44648 l 10.422639,-0.98001 c 0.202073,2.10863 0.510849,4.19939 0.920523,6.26647 M 48.841442,87.977355 35.45203,83.350737 c -0.681703,1.974941 -1.271974,3.981442 -1.765121,6.013808 L 23.560667,86.910516 c 2.012874,-2.010272 4.09749,-3.84604 6.235715,-5.514049 -0.652983,-2.647928 -1.164004,-5.389674 -1.526268,-8.206834 l 9.512158,4.299981 c -0.865485,1.917873 -1.644168,3.873515 -2.330242,5.861123 M 63.036054,65.779324 52.923915,55.680104 c -1.484755,1.486662 -2.907971,3.034776 -4.263954,4.638646 l -8.022117,-6.786527 c 2.679306,-0.864613 5.356928,-1.568365 8.014735,-2.117999 0.55648,-2.668825 1.268458,-5.357379 2.14273,-8.047259 l 6.792631,8.017078 c -1.613137,1.365909 -3.169746,2.799864 -4.664025,4.296061 m 32.293904,-4.126253 -4.646,-13.382957 c -1.973961,0.684536 -3.916275,1.460414 -5.821248,2.321942 l -4.307807,-9.508842 c 2.803352,0.366354 5.531596,0.879929 8.166599,1.533979 1.678621,-2.145324 3.526276,-4.236289 5.549761,-6.25449 l 2.462921,10.123991 c -2.044928,0.498086 -4.063604,1.094494 -6.050226,1.78342 m 29.887441,11.502937 2.72512,-13.909746 c -2.05463,-0.403974 -4.13255,-0.708242 -6.22806,-0.907113 l 0.97034,-10.424118 c 2.3123,1.732578 4.44632,3.557221 6.4088,5.455796 2.54595,-1.003175 5.20623,-1.868133 7.96242,-2.588081 l -2.98663,9.980368 c -2.01483,-0.602736 -4.05906,-1.110288 -6.12687,-1.516852 m 20.80621,25.020246 8.80357,-11.238316 c -1.65323,-1.293895 -3.35963,-2.517977 -5.11351,-3.666552 l 5.74851,-8.765666 c 1.19026,2.549134 2.21587,5.116907 3.08359,7.685187 2.71753,0.230458 5.4753,0.612669 8.25489,1.153426 l -7.1494,7.706514 c -1.55029,-1.437811 -3.16026,-2.810716 -4.82408,-4.112909 m 8.1233,31.704074 12.69315,-6.378023 c -0.93796,-1.869969 -1.959,-3.700016 -3.05741,-5.484449 l 8.91651,-5.497031 c -0.0171,2.823304 -0.1852,5.591822 -0.49755,8.287423 2.34471,1.376769 4.65556,2.918826 6.91416,4.632966 l -9.70199,3.822478 c -0.7699,-1.957028 -1.62974,-3.879425 -2.57372,-5.761387" />
<path
id="path5883"
d="m 199.93659,100.06735 -5.34837,9.59432 h -12.75166"
style="fill:none;stroke:#000000;stroke-width:0.730286px;stroke-linecap:butt;stroke-linejoin:miter;stroke-opacity:1" />
</g>
</g>
</svg>

Loading…
Cancel
Save