diff --git a/chapters/1-introduction.tex b/chapters/1-introduction.tex
index 6369ae0..a6c4eec 100644
--- a/chapters/1-introduction.tex
+++ b/chapters/1-introduction.tex
@@ -134,7 +134,7 @@ di veicolare informazioni e
 sostanze tra una cellula e l'altra, guidare la loro proliferazione o migrazione, mantenere la stabilità dei tessuti o avviarne la
 riparazione quando necessario.
 
-\begin{figure}
+\begin{figure}[ht]
     \centering
     \includegraphics[width=0.5\linewidth]{images/adjunc.pdf}
     \caption{Sequenza di cellule connesse da \emph{giunzioni aderenti} (sopra) e dettaglio di una giunzione aderente, con indicazione delle principali proteine coinvolte (sotto)}
@@ -189,7 +189,7 @@ Lo stato attuale delle conoscenze sulla rete di interazioni che governa e
 regola il funzionamento delle giunzioni aderenti è riportato in Appendice,
 sotto forma di diagramma delle vie di segnalazione.
 
-\begin{figure}
+\begin{figure}[ht]
     \centering
     \includegraphics{images/aj.pdf}
     \caption{Ruolo di \textbf{caderina} e catenine nelle \textit{giunzioni aderenti}}
@@ -209,7 +209,7 @@ che modulano e regolano il loro funzionamento.
 Come nel caso delle giunzioni aderenti questo emerge dall'interazione
 tra un certo numero di proteine interagenti.
 
-\begin{figure}
+\begin{figure}[ht]
     \centering
     \includegraphics{images/tj.pdf}
     \caption{Ruolo di \textbf{ZO-1} nelle \emph{giunzioni occludenti}}
@@ -281,9 +281,16 @@ covalentemente molecole di \textit{streptavidina} alla loro superificie.
 In questo modo è possibile successivamente ottenere il legame delle
 microsfere col polimero biologico d'interesse, purché esso sia stato
 preventivamente biotilinato. Si sfrutta in questo modo il legame 
-streptavidina-biotina, estremamente stabile e praticamente irreversibile.
-
+streptavidina-biotina, estremamente stabile e praticamente irreversibile
+(vedi figura \ref{fig:biotin-streptavidin}).
 
+\begin{figure}[ht]
+    \centering
+    \includegraphics[width=0.5\linewidth]{images/biotin-streptavidin.pdf}
+    \caption{Manipolazione di una proteina target utilizzando una
+    microsfera intrappolata e il legame biotina-streptavidina.}
+    \label{fig:biotin-streptavidin}
+\end{figure}
 
 Per descrivere quantitativamente il funzionamento delle pinzette
 ottiche consideriamo in generale l'effetto dell'interazione tra
@@ -394,10 +401,4 @@ Il valore di $k$ per una certa trappola ottica, come vedremo, può essere
 determinato attraverso un'apposita procedura di calibrazione che sfrutta
 la diffusione della microsfera all'interno della trappola.
 
-
-
-
-
-\section{Spettroscopia force-clamp}
-
-\section{\textit{Imaging} di singola molecola}
\ No newline at end of file
+\section{Microscopia di fluorescenza}
\ No newline at end of file
diff --git a/chapters/2-methods.tex b/chapters/2-methods.tex
index 0b37b07..83646ce 100644
--- a/chapters/2-methods.tex
+++ b/chapters/2-methods.tex
@@ -1,6 +1,9 @@
  %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%% %%%%%%%%%
 \chapter{Metodi}
 
+\section{Force-clamp}
+
+
 In figura \ref{fig:setup} è mostrato uno schema integrale
 dell'apparato realizzato.
 Le componenti principali sono descritte nella sezione \ref{sec:setup}.
diff --git a/images/biotin-streptavidin.pdf b/images/biotin-streptavidin.pdf
new file mode 100644
index 0000000..b81b408
Binary files /dev/null and b/images/biotin-streptavidin.pdf differ
diff --git a/images_src/biotin-streptavidin.svg b/images_src/biotin-streptavidin.svg
new file mode 100644
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