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@ -1,9 +1,9 @@ |
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# -*- coding: utf-8 -*- |
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# -*- coding: utf-8 -*- |
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from nd2reader.model import Image, ImageGroup |
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from nd2reader.model import ImageGroup |
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from nd2reader.driver import get_driver |
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from nd2reader.driver import get_driver |
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from nd2reader.driver.version import get_version |
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from nd2reader.driver.version import get_version |
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import six |
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import warnings |
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class Nd2(object): |
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class Nd2(object): |
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@ -12,13 +12,14 @@ class Nd2(object): |
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""" |
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""" |
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def __init__(self, filename): |
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def __init__(self, filename): |
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self._filename = filename |
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version = get_version(filename) |
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version = get_version(filename) |
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self._driver = get_driver(filename, version) |
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self._driver = get_driver(filename, version) |
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self._metadata = self._driver.get_metadata() |
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self._metadata = self._driver.get_metadata() |
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def __repr__(self): |
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def __repr__(self): |
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return "\n".join(["<ND2 %s>" % self._driver._filename, |
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"Created: %s" % self._driver.absolute_start, |
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return "\n".join(["<ND2 %s>" % self._filename, |
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"Created: %s" % self.date, |
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"Image size: %sx%s (HxW)" % (self.height, self.width), |
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"Image size: %sx%s (HxW)" % (self.height, self.width), |
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"Frames: %s" % len(self.frames), |
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"Frames: %s" % len(self.frames), |
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"Channels: %s" % ", ".join(["'%s'" % str(channel) for channel in self.channels]), |
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"Channels: %s" % ", ".join(["'%s'" % str(channel) for channel in self.channels]), |
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@ -43,32 +44,18 @@ class Nd2(object): |
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>>> nd2 = Nd2("my_images.nd2") |
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>>> nd2 = Nd2("my_images.nd2") |
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>>> image = nd2[16] # gets 17th frame |
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>>> image = nd2[16] # gets 17th frame |
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>>> for image in nd2[100:200]: # iterate over the 100th to 200th images |
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>>> for image in nd2[100:200]: # iterate over the 100th to 200th images |
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>>> do_something(image.data) |
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>>> do_something(image) |
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>>> for image in nd2[::-1]: # iterate backwards |
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>>> for image in nd2[::-1]: # iterate backwards |
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>>> do_something(image.data) |
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>>> do_something(image) |
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>>> for image in nd2[37:422:17]: # do something super weird if you really want to |
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>>> for image in nd2[37:422:17]: # do something super weird if you really want to |
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>>> do_something(image.data) |
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>>> do_something(image) |
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:type item: int or slice |
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:type item: int or slice |
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:rtype: nd2reader.model.Image() or generator |
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:rtype: nd2reader.model.Image() or generator |
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""" |
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""" |
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if isinstance(item, int): |
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if isinstance(item, int): |
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try: |
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channel_offset = item % len(self.channels) |
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fov = self._calculate_field_of_view(item) |
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channel = self._calculate_channel(item) |
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z_level = self._calculate_z_level(item) |
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image_group_number = int(item / len(self.channels)) |
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frame_number = self._calculate_frame_number(image_group_number, fov, z_level) |
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timestamp, raw_image_data = self._get_raw_image_data(image_group_number, channel_offset) |
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image = Image(timestamp, frame_number, raw_image_data, fov, channel, z_level, self.height, self.width) |
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except (TypeError, ValueError): |
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return None |
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except KeyError: |
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raise IndexError("Invalid frame number.") |
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else: |
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return image |
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return self._driver.get_image(item) |
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elif isinstance(item, slice): |
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elif isinstance(item, slice): |
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return self._slice(item.start, item.stop, item.step) |
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return self._slice(item.start, item.stop, item.step) |
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raise IndexError |
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raise IndexError |
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@ -80,7 +67,7 @@ class Nd2(object): |
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:type start: int |
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:type start: int |
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:type stop: int |
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:type stop: int |
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:type step: int |
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:type step: int |
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:rtype: nd2reader.model.Image() or None |
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:rtype: nd2reader.model.Image() |
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""" |
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""" |
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start = start if start is not None else 0 |
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start = start if start is not None else 0 |
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@ -101,15 +88,37 @@ class Nd2(object): |
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:return: model.ImageSet() |
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:return: model.ImageSet() |
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""" |
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""" |
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for time_index in self.time_indexes: |
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image_set = ImageGroup() |
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warnings.warn("nd2.image_sets will be removed from the nd2reader library in the near future.", DeprecationWarning) |
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for frame in self.frames: |
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image_group = ImageGroup() |
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for fov in self.fields_of_view: |
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for fov in self.fields_of_view: |
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for channel_name in self.channels: |
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for channel_name in self.channels: |
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for z_level in self.z_levels: |
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for z_level in self.z_levels: |
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image = self.get_image(time_index, fov, channel_name, z_level) |
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image = self.get_image(frame, fov, channel_name, z_level) |
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if image is not None: |
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if image is not None: |
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image_set.add(image) |
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yield image_set |
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image_group.add(image) |
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yield image_group |
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@property |
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def date(self): |
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return self._metadata.date |
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@property |
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def z_levels(self): |
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return self._metadata.z_levels |
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@property |
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def fields_of_view(self): |
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return self._metadata.fields_of_view |
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@property |
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def channels(self): |
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return self._metadata.channels |
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@property |
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def frames(self): |
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return self._metadata.frames |
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@property |
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@property |
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def height(self): |
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def height(self): |
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@ -118,7 +127,7 @@ class Nd2(object): |
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:rtype: int |
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:rtype: int |
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""" |
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""" |
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return self.metadata[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiHeight')] |
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return self._metadata.height |
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@property |
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@property |
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def width(self): |
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def width(self): |
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@ -127,12 +136,11 @@ class Nd2(object): |
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:rtype: int |
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:rtype: int |
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""" |
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""" |
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return self.metadata[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiWidth')] |
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return self._metadata.width |
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def get_image(self, frame_number, field_of_view, channel_name, z_level): |
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def get_image(self, frame_number, field_of_view, channel_name, z_level): |
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""" |
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""" |
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Returns an Image if data exists for the given parameters, otherwise returns None. In general, you should avoid |
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using this method unless you're very familiar with the structure of ND2 files. |
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Returns an Image if data exists for the given parameters, otherwise returns None. |
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:type frame_number: int |
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:type frame_number: int |
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:param field_of_view: the label for the place in the XY-plane where this image was taken. |
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:param field_of_view: the label for the place in the XY-plane where this image was taken. |
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@ -141,14 +149,8 @@ class Nd2(object): |
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:type channel_name: str |
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:type channel_name: str |
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:param z_level: the label for the location in the Z-plane where this image was taken. |
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:param z_level: the label for the location in the Z-plane where this image was taken. |
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:type z_level: int |
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:type z_level: int |
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:rtype: nd2reader.model.Image() or None |
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:rtype: nd2reader.model.Image() |
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""" |
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""" |
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image_group_number = self._calculate_image_group_number(frame_number, field_of_view, z_level) |
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try: |
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timestamp, raw_image_data = self._get_raw_image_data(image_group_number, self._channel_offset[channel_name]) |
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image = Image(timestamp, frame_number, raw_image_data, field_of_view, channel_name, z_level, self.height, self.width) |
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except TypeError: |
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return None |
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else: |
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return image |
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return self._driver.get_image_by_attributes(frame_number, field_of_view, channel_name, z_level) |