@ -1,15 +0,0 @@ | |||
engines: | |||
duplication: | |||
enabled: true | |||
config: | |||
languages: | |||
- python | |||
fixme: | |||
enabled: true | |||
radon: | |||
enabled: true | |||
ratings: | |||
paths: | |||
- "**.py" | |||
exclude_paths: | |||
- tests/ |
@ -0,0 +1,40 @@ | |||
name: Build | |||
on: | |||
push: | |||
branches: [ master ] | |||
pull_request: | |||
branches: [ master ] | |||
jobs: | |||
build: | |||
runs-on: ubuntu-latest | |||
strategy: | |||
fail-fast: false | |||
matrix: | |||
python-version: [3.7, 3.8, 3.9] | |||
steps: | |||
- uses: actions/checkout@v2 | |||
- name: Set up Python ${{ matrix.python-version }} | |||
uses: actions/setup-python@v2 | |||
with: | |||
python-version: ${{ matrix.python-version }} | |||
- name: Install dependencies | |||
run: | | |||
python -m pip install --upgrade pip | |||
python -m pip install flake8 pytest | |||
if [ -f requirements.txt ]; then pip install -r requirements.txt; fi | |||
- name: Lint with flake8 | |||
run: | | |||
# stop the build if there are Python syntax errors or undefined names | |||
flake8 . --count --select=E9,F63,F7,F82 --show-source --statistics | |||
# exit-zero treats all errors as warnings. The GitHub editor is 127 chars wide | |||
flake8 . --count --exit-zero --max-complexity=10 --max-line-length=127 --statistics | |||
- name: Install as package | |||
run: | | |||
python setup.py develop | |||
- name: Test with pytest | |||
run: | | |||
pytest |
@ -1,4 +1,4 @@ | |||
[submodule "docs"] | |||
path = docs | |||
url = https://github.com/rbnvrw/nd2reader.git | |||
url = git@github.com:rbnvrw/nd2reader.git | |||
branch = gh-pages |
@ -1,33 +0,0 @@ | |||
env: | |||
global: | |||
- CC_TEST_REPORTER_ID=8582900c285e4da0f253555b1bac1ba501bd6ff07850b0f227166b3cdac59ecc | |||
language: python | |||
git: | |||
depth: 3 | |||
notifications: | |||
email: false | |||
python: | |||
- 3.5 | |||
- 3.6 | |||
- 3.7 | |||
- 3.8 | |||
install: | |||
- pip install --upgrade pip setuptools wheel | |||
- pip install --only-binary=numpy numpy | |||
- pip install -r requirements.txt | |||
- pip install 'coverage>=4.0,<4.4' --force-reinstall # Upstream bug: https://github.com/nedbat/coveragepy/issues/578 | |||
before_script: | |||
- curl -L https://codeclimate.com/downloads/test-reporter/test-reporter-latest-linux-amd64 > ./cc-test-reporter | |||
- chmod +x ./cc-test-reporter | |||
- ./cc-test-reporter before-build | |||
script: python ./test.py | |||
after_script: | |||
- ./cc-test-reporter after-build --exit-code $TRAVIS_TEST_RESULT |
@ -1,6 +1,6 @@ | |||
include MANIFEST.in | |||
include VERSION | |||
include setup.py | |||
include setup.cfg | |||
include README.md | |||
include LICENSE | |||
include COPYING |
@ -1 +1 @@ | |||
Subproject commit f700c239f8f9d7d1f99a3c10d9f67e2b3b8ef307 | |||
Subproject commit 42dbfdf18e0ed7656ef73ac66e889c3138ef756f |
@ -1,4 +1,4 @@ | |||
numpy>=1.9.2 | |||
numpy>=1.14 | |||
six>=1.4 | |||
xmltodict>=0.9.2 | |||
pims>=0.3.0 | |||
pims>=0.3.0 |
@ -1,119 +0,0 @@ | |||
# Tutorial | |||
### Installation | |||
The package is available on PyPi. Install it using: | |||
``` | |||
pip install nd2reader | |||
``` | |||
If you don't already have the packages `numpy`, `pims`, `six` and `xmltodict`, they will be installed automatically if you use the `setup.py` script. | |||
`nd2reader` is an order of magnitude faster in Python 3. I recommend using it unless you have no other choice. Python 2.7 and Python >= 3.4 are supported. | |||
#### Installation via Conda Forge | |||
Installing `nd2reader` from the `conda-forge` channel can be achieved by adding `conda-forge` to your channels with: | |||
``` | |||
conda config --add channels conda-forge | |||
``` | |||
Once the `conda-forge` channel has been enabled, `nd2reader` can be installed with: | |||
``` | |||
conda install nd2reader | |||
``` | |||
It is possible to list all of the versions of `nd2reader` available on your platform with: | |||
``` | |||
conda search nd2reader --channel conda-forge | |||
``` | |||
### Opening ND2s | |||
`nd2reader` follows the [pims](https://github.com/soft-matter/pims) framework. To open a file and show the first frame: | |||
```python | |||
from nd2reader import ND2Reader | |||
import matplotlib.pyplot as plt | |||
with ND2Reader('my_directory/example.nd2') as images: | |||
plt.imshow(images[0]) | |||
``` | |||
After opening the file, all `pims` features are supported. Please refer to the [pims documentation](http://soft-matter.github.io/pims/). | |||
### ND2 metadata | |||
The ND2 file contains various metadata, such as acquisition information, | |||
regions of interest and custom user comments. Most of this metadata is parsed | |||
and available in dictionary form. For example: | |||
```python | |||
from nd2reader import ND2Reader | |||
with ND2Reader('my_directory/example.nd2') as images: | |||
# width and height of the image | |||
print('%d x %d px' % (images.metadata['width'], images.metadata['height'])) | |||
``` | |||
All metadata properties are: | |||
* `width`: the width of the image in pixels | |||
* `height`: the height of the image in pixels | |||
* `date`: the date the image was taken | |||
* `fields_of_view`: the fields of view in the image | |||
* `frames`: a list of all frame numbers | |||
* `z_levels`: the z levels in the image | |||
* `total_images_per_channel`: the number of images per color channel | |||
* `channels`: the color channels | |||
* `pixel_microns`: the amount of microns per pixel | |||
* `rois`: the regions of interest (ROIs) defined by the user | |||
* `experiment`: information about the nature and timings of the ND experiment | |||
### Iterating over fields of view | |||
Using `NDExperiments` in the Nikon software, it is possible to acquire images on different `(x, y)` positions. | |||
This is referred to as different fields of view. Using this reader, the fields of view are on the `v` axis. | |||
For example: | |||
```python | |||
from nd2reader import ND2Reader | |||
with ND2Reader('my_directory/example.nd2') as images: | |||
# width and height of the image | |||
print(images.metadata) | |||
``` | |||
will output | |||
```python | |||
{'channels': ['BF100xoil-1x-R', 'BF+RITC'], | |||
'date': datetime.datetime(2017, 10, 30, 14, 35, 18), | |||
'experiment': {'description': 'ND Acquisition', | |||
'loops': [{'duration': 0, | |||
'sampling_interval': 0.0, | |||
'start': 0, | |||
'stimulation': False}]}, | |||
'fields_of_view': [0, 1], | |||
'frames': [0], | |||
'height': 1895, | |||
'num_frames': 1, | |||
'pixel_microns': 0.09214285714285715, | |||
'total_images_per_channel': 6, | |||
'width': 2368, | |||
'z_levels': [0, 1, 2]} | |||
``` | |||
for our example file. As you can see from the metadata, it has two fields of view. We can also look at the sizes of the axes: | |||
```python | |||
print(images.sizes) | |||
``` | |||
```python | |||
{'c': 2, 't': 1, 'v': 2, 'x': 2368, 'y': 1895, 'z': 3} | |||
``` | |||
As you can see, the fields of view are listed on the `v` axis. It is therefore possible to loop over them like this: | |||
```python | |||
images.iter_axes = 'v' | |||
for fov in images: | |||
print(fov) # Frame containing one field of view | |||
``` | |||
For more information on axis bundling and iteration, refer to the [pims documentation](http://soft-matter.github.io/pims/v0.4/multidimensional.html#axes-bundling). |
@ -0,0 +1,148 @@ | |||
Tutorial | |||
======== | |||
Installation | |||
~~~~~~~~~~~~ | |||
The package is available on PyPi. Install it using: | |||
:: | |||
pip install nd2reader | |||
If you don't already have the packages ``numpy``, ``pims``, ``six`` and | |||
``xmltodict``, they will be installed automatically if you use the | |||
``setup.py`` script. ``nd2reader`` is an order of magnitude faster in | |||
Python 3. I recommend using it unless you have no other choice. Python | |||
2.7 and Python >= 3.4 are supported. | |||
Installation via Conda Forge | |||
^^^^^^^^^^^^^^^^^^^^^^^^^^^^ | |||
Installing ``nd2reader`` from the ``conda-forge`` channel can be | |||
achieved by adding ``conda-forge`` to your channels with: | |||
:: | |||
conda config --add channels conda-forge | |||
Once the ``conda-forge`` channel has been enabled, ``nd2reader`` can be | |||
installed with: | |||
:: | |||
conda install nd2reader | |||
It is possible to list all of the versions of ``nd2reader`` available on | |||
your platform with: | |||
:: | |||
conda search nd2reader --channel conda-forge | |||
Opening ND2s | |||
~~~~~~~~~~~~ | |||
``nd2reader`` follows the `pims <https://github.com/soft-matter/pims>`__ | |||
framework. To open a file and show the first frame: | |||
.. code:: python | |||
from nd2reader import ND2Reader | |||
import matplotlib.pyplot as plt | |||
with ND2Reader('my_directory/example.nd2') as images: | |||
plt.imshow(images[0]) | |||
After opening the file, all ``pims`` features are supported. Please | |||
refer to the `pims | |||
documentation <http://soft-matter.github.io/pims/>`__. | |||
ND2 metadata | |||
~~~~~~~~~~~~ | |||
The ND2 file contains various metadata, such as acquisition information, | |||
regions of interest and custom user comments. Most of this metadata is | |||
parsed and available in dictionary form. For example: | |||
.. code:: python | |||
from nd2reader import ND2Reader | |||
with ND2Reader('my_directory/example.nd2') as images: | |||
# width and height of the image | |||
print('%d x %d px' % (images.metadata['width'], images.metadata['height'])) | |||
All metadata properties are: | |||
- ``width``: the width of the image in pixels | |||
- ``height``: the height of the image in pixels | |||
- ``date``: the date the image was taken | |||
- ``fields_of_view``: the fields of view in the image | |||
- ``frames``: a list of all frame numbers | |||
- ``z_levels``: the z levels in the image | |||
- ``total_images_per_channel``: the number of images per color channel | |||
- ``channels``: the color channels | |||
- ``pixel_microns``: the amount of microns per pixel | |||
- ``rois``: the regions of interest (ROIs) defined by the user | |||
- ``experiment``: information about the nature and timings of the ND | |||
experiment | |||
Iterating over fields of view | |||
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ | |||
Using ``NDExperiments`` in the Nikon software, it is possible to acquire | |||
images on different ``(x, y)`` positions. This is referred to as | |||
different fields of view. Using this reader, the fields of view are on | |||
the ``v`` axis. For example: | |||
.. code:: python | |||
from nd2reader import ND2Reader | |||
with ND2Reader('my_directory/example.nd2') as images: | |||
# width and height of the image | |||
print(images.metadata) | |||
will output | |||
.. code:: python | |||
{'channels': ['BF100xoil-1x-R', 'BF+RITC'], | |||
'date': datetime.datetime(2017, 10, 30, 14, 35, 18), | |||
'experiment': {'description': 'ND Acquisition', | |||
'loops': [{'duration': 0, | |||
'sampling_interval': 0.0, | |||
'start': 0, | |||
'stimulation': False}]}, | |||
'fields_of_view': [0, 1], | |||
'frames': [0], | |||
'height': 1895, | |||
'num_frames': 1, | |||
'pixel_microns': 0.09214285714285715, | |||
'total_images_per_channel': 6, | |||
'width': 2368, | |||
'z_levels': [0, 1, 2]} | |||
for our example file. As you can see from the metadata, it has two | |||
fields of view. We can also look at the sizes of the axes: | |||
.. code:: python | |||
print(images.sizes) | |||
.. code:: python | |||
{'c': 2, 't': 1, 'v': 2, 'x': 2368, 'y': 1895, 'z': 3} | |||
As you can see, the fields of view are listed on the ``v`` axis. It is | |||
therefore possible to loop over them like this: | |||
.. code:: python | |||
images.iter_axes = 'v' | |||
for fov in images: | |||
print(fov) # Frame containing one field of view | |||
For more information on axis bundling and iteration, refer to the `pims | |||
documentation <http://soft-matter.github.io/pims/v0.4/multidimensional.html#axes-bundling>`__. |