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160 lines
4.5 KiB
160 lines
4.5 KiB
from pims import FramesSequenceND, Frame
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from nd2reader.parser import Parser
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import numpy as np
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class ND2Reader(FramesSequenceND):
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"""PIMS wrapper for the ND2 parser.
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This is the main class: use this to process your .nd2 files.
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"""
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def __init__(self, filename):
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super(self.__class__, self).__init__()
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self.filename = filename
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# first use the parser to parse the file
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self._fh = open(filename, "rb")
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self._parser = Parser(self._fh)
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# Setup metadata
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self.metadata = self._parser.metadata
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# Set data type
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self._dtype = self._parser.get_dtype_from_metadata()
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# Setup the axes
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self._setup_axes()
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@classmethod
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def class_exts(cls):
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"""Let PIMS open function use this reader for opening .nd2 files
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"""
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return {'nd2'} | super(ND2Reader, cls).class_exts()
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def close(self):
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"""Correctly close the file handle
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"""
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if self._fh is not None:
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self._fh.close()
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def get_frame(self, i):
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"""Return one frame
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Args:
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i: The frame number
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Returns:
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numpy.ndarray: The requested frame
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"""
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fetch_all_channels = 'c' in self.bundle_axes
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if fetch_all_channels:
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return self._get_frame_all_channels(i)
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else:
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return self.get_frame_2D(self.default_coords['c'], i, self.default_coords['z'])
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def _get_frame_all_channels(self, i):
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"""Get all color channels for this frame
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Args:
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i: The frame number
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Returns:
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numpy.ndarray: The requested frame, with all color channels.
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"""
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frames = None
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for c in range(len(self.metadata["channels"])):
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frame = self.get_frame_2D(c, i, self.default_coords['z'])
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if frames is None:
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frames = Frame([frame])
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else:
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frames = np.concatenate((frames, [frame]), axis=0)
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return frames
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def get_frame_2D(self, c, t, z):
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"""Gets a given frame using the parser
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Args:
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c: The color channel number
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t: The frame number
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z: The z stack number
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Returns:
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numpy.ndarray: The requested frame
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"""
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c_name = self.metadata["channels"][c]
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return self._parser.get_image_by_attributes(t, 0, c_name, z, self.metadata["height"], self.metadata["width"])
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@property
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def parser(self):
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"""
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Returns the parser object.
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Returns:
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Parser: the parser object
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"""
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return self._parser
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@property
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def pixel_type(self):
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"""Return the pixel data type
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Returns:
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dtype: the pixel data type
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"""
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return self._dtype
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def _get_metadata_property(self, key, default=None):
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if self.metadata is None:
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return default
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if key not in self.metadata:
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return default
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if self.metadata[key] is None:
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return default
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return self.metadata[key]
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def _setup_axes(self):
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"""Setup the xyctz axes, iterate over t axis by default
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"""
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self._init_axis('x', self._get_metadata_property("width", default=0))
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self._init_axis('y', self._get_metadata_property("height", default=0))
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self._init_axis('c', len(self._get_metadata_property("channels", default=[])))
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self._init_axis('t', len(self._get_metadata_property("frames", default=[])))
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self._init_axis('z', len(self._get_metadata_property("z_levels", default=[])))
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# provide the default
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self.iter_axes = 't'
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def get_timesteps(self):
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"""Get the timesteps of the experiment
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Returns:
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np.ndarray: an array of times in milliseconds.
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"""
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timesteps = np.array([])
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current_time = 0.0
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for loop in self.metadata['experiment']['loops']:
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if loop['stimulation']:
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continue
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if loop['sampling_interval'] == 0:
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# This is a loop were no data is acquired
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current_time += loop['duration']
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continue
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timesteps = np.concatenate(
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(timesteps, np.arange(current_time, current_time + loop['duration'], loop['sampling_interval'])))
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current_time += loop['duration']
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# if experiment did not finish, number of timesteps is wrong. Take correct amount of leading timesteps.
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return timesteps[:self.metadata['num_frames']]
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