import numpy as np
|
|
import logging
|
|
|
|
log = logging.getLogger(__name__)
|
|
|
|
|
|
class ImageSet(object):
|
|
"""
|
|
A group of images that share the same timestamp. NIS Elements doesn't store a unique timestamp for every
|
|
image, rather, it stores one for each set of images that share the same field of view and z-axis level.
|
|
|
|
"""
|
|
def __init__(self):
|
|
self._images = []
|
|
|
|
def add(self, image):
|
|
"""
|
|
:type image: nd2reader.model.Image()
|
|
|
|
"""
|
|
self._images.append(image)
|
|
|
|
def __iter__(self):
|
|
for image in self._images:
|
|
yield image
|
|
|
|
|
|
class Image(object):
|
|
def __init__(self, timestamp, raw_array, field_of_view, channel, z_level, height, width):
|
|
self._timestamp = timestamp
|
|
self._raw_data = raw_array
|
|
self._field_of_view = field_of_view
|
|
self._channel = channel
|
|
self._z_level = z_level
|
|
self._height = height
|
|
self._width = width
|
|
self._data = None
|
|
|
|
@property
|
|
def field_of_view(self):
|
|
return self._field_of_view
|
|
|
|
@property
|
|
def timestamp(self):
|
|
"""
|
|
The number of seconds after the beginning of the acquisition that the image was taken. Note that for a given field
|
|
of view and z-level offset, if you have images of multiple channels, they will all be given the same timestamp.
|
|
No, this doesn't make much sense. But that's how ND2s are structured, so if your experiment depends on millisecond
|
|
accuracy, you need to find an alternative imaging system.
|
|
|
|
"""
|
|
return self._timestamp / 1000.0
|
|
|
|
@property
|
|
def channel(self):
|
|
return self._channel
|
|
|
|
@property
|
|
def z_level(self):
|
|
return self._z_level
|
|
|
|
@property
|
|
def data(self):
|
|
if self._data is None:
|
|
# The data is just a flat, 1-dimensional array. We convert it to a 2D image here.
|
|
self._data = np.reshape(self._raw_data, (self._height, self._width))
|
|
return self._data
|
|
|
|
@property
|
|
def is_valid(self):
|
|
return np.any(self._raw_data)
|