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# -*- coding: utf-8 -*-
import logging
from nd2reader.model import Image, ImageSet
from nd2reader.parser import Nd2Parser
log = logging.getLogger(__name__)
log.addHandler(logging.StreamHandler())
log.setLevel(logging.WARNING)
class Nd2(Nd2Parser):
def __init__(self, filename):
super(Nd2, self).__init__(filename)
self._filename = filename
def __repr__(self):
return "\n".join(["ND2: %s" % self._filename,
"Created: %s" % self._absolute_start.strftime("%Y-%m-%d %H:%M:%S"),
"Image size: %sx%s (HxW)" % (self.height, self.width),
"Image cycles: %s" % self._time_index_count,
"Channels: %s" % ", ".join(["'%s'" % channel for channel in self._channels]),
"Fields of View: %s" % self._field_of_view_count,
"Z-Levels: %s" % self._z_level_count
])
def __iter__(self):
for i in range(self._image_count):
for fov in range(self._field_of_view_count):
for z_level in range(self._z_level_count):
for channel_name in self._channels:
image = self.get_image(i, fov, channel_name, z_level)
if image is not None:
yield image
@property
def image_sets(self):
for time_index in xrange(self._time_index_count):
image_set = ImageSet()
for fov in range(self._field_of_view_count):
for channel_name in self._channels:
for z_level in xrange(self._z_level_count):
image = self.get_image(time_index, fov, channel_name, z_level)
if image is not None:
image_set.add(image)
yield image_set
def get_image(self, time_index, fov, channel_name, z_level):
image_set_number = self._calculate_image_set_number(time_index, fov, z_level)
try:
timestamp, raw_image_data = self._get_raw_image_data(image_set_number, self._channel_offset[channel_name])
image = Image(timestamp, raw_image_data, fov, channel_name, z_level, self.height, self.width)
except TypeError:
return None
else:
return image
@property
def height(self):
"""
:return: height of each image, in pixels
"""
return self.metadata['ImageAttributes']['SLxImageAttributes']['uiHeight']
@property
def width(self):
"""
:return: width of each image, in pixels
"""
return self.metadata['ImageAttributes']['SLxImageAttributes']['uiWidth']