testtest/sphinx/tutorial.md

Tutorial

Installation

For now, the package is only available via GitHub. Install it using:

pip install --upgrade https://github.com/rbnvrw/nd2reader/tarball/master

If you don't already have the packages numpy, pims, six and xmltodict, they will be installed automatically if you use the setup.py script. nd2reader is an order of magnitude faster in Python 3. I recommend using it unless you have no other choice. Python 2.7 and Python >= 3.4 are supported.

Opening ND2s

nd2reader follows the pims framework. To open a file and show the first frame:

from nd2reader import ND2Reader
import matplotlib.pyplot as plt

with ND2Reader('my_directory/example.nd2') as images:
  plt.imshow(images[0])

After opening the file, all pims features are supported. Please refer to the pims documentation.

ND2 metadata

The ND2 file contains various metadata, such as acquisition information, regions of interest and custom user comments. Most of this metadata is parsed and available in dictionary form. For example:

from nd2reader import ND2Reader

with ND2Reader('my_directory/example.nd2') as images:
    # width and height of the image
    print('%d x %d px' % (images.metadata['width'], images.metadata['height']))

All metadata properties are:

• width: the width of the image in pixels
• height: the height of the image in pixels
• date: the date the image was taken
• fields_of_view: the fields of view in the image
• frames: a list of all frame numbers
• z_levels: the z levels in the image
• total_images_per_channel: the number of images per color channel
• channels: the color channels
• pixel_microns: the amount of microns per pixel
• rois: the regions of interest (ROIs) defined by the user
• experiment: information about the nature and timings of the ND experiment