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176 lines
5.8 KiB
176 lines
5.8 KiB
# -*- coding: utf-8 -*-
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from nd2reader.parser import get_parser
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from nd2reader.version import get_version
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class Nd2(object):
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""" Allows easy access to NIS Elements .nd2 image files. """
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def __init__(self, filename):
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self._filename = filename
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self._fh = open(filename, "rb")
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major_version, minor_version = get_version(self._fh)
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self._parser = get_parser(self._fh, major_version, minor_version)
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self._driver = self._parser.driver
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self._metadata = self._parser.metadata
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def __enter__(self):
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return self
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def __exit__(self, exc_type, exc_val, exc_tb):
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if self._fh is not None:
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self._fh.close()
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def __repr__(self):
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return "\n".join(["<ND2 %s>" % self._filename,
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"Created: %s" % (self.date if self.date is not None else "Unknown"),
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"Image size: %sx%s (HxW)" % (self.height, self.width),
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"Frames: %s" % len(self.frames),
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"Channels: %s" % ", ".join(["'%s'" % str(channel) for channel in self.channels]),
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"Fields of View: %s" % len(self.fields_of_view),
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"Z-Levels: %s" % len(self.z_levels)
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])
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def __len__(self):
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"""
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This should be the total number of images in the ND2, but it may be inaccurate. If the ND2 contains a
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different number of images in a cycle (i.e. there are "gap" images) it will be higher than reality.
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:rtype: int
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"""
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return self._metadata.total_images_per_channel * len(self.channels)
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def __getitem__(self, item):
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"""
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Allows slicing ND2s.
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:type item: int or slice
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:rtype: nd2reader.model.Image() or generator
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"""
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if isinstance(item, int):
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try:
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image = self._driver.get_image(item)
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except KeyError:
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raise IndexError
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else:
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return image
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elif isinstance(item, slice):
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return self._slice(item.start, item.stop, item.step)
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raise IndexError
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def _slice(self, start, stop, step):
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"""
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Allows for iteration over a selection of the entire dataset.
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:type start: int
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:type stop: int
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:type step: int
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:rtype: nd2reader.model.Image()
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"""
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start = start if start is not None else 0
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step = step if step is not None else 1
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stop = stop if stop is not None else len(self)
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# This weird thing with the step allows you to iterate backwards over the images
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for i in range(start, stop)[::step]:
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yield self[i]
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@property
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def date(self):
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"""
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The date and time that the acquisition began. Not guaranteed to have been recorded.
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:rtype: datetime.datetime() or None
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"""
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return self._metadata.date
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@property
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def z_levels(self):
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"""
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A list of integers that represent the different levels on the Z-axis that images were taken. Currently this is just a list of numbers from 0 to N.
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For example, an ND2 where images were taken at -3µm, 0µm, and +5µm from a set position would be represented by 0, 1 and 2, respectively. ND2s do store the actual
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offset of each image in micrometers and in the future this will hopefully be available. For now, however, you will have to match up the order yourself.
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:return: list of int
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"""
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return self._metadata.z_levels
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@property
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def fields_of_view(self):
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"""
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A list of integers representing the various stage locations, in the order they were taken in the first round of acquisition.
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:return: list of int
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"""
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return self._metadata.fields_of_view
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@property
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def channels(self):
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"""
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A list of channel (i.e. wavelength) names. These are set by the user in NIS Elements.
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:return: list of str
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"""
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return self._metadata.channels
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@property
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def frames(self):
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"""
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A list of integers representing groups of images. ND2s consider images to be part of the same frame if they are in the same field of view and don't have the same channel.
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So if you take a bright field and GFP image at four different fields of view over and over again, you'll have 8 images and 4 frames per cycle.
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:return: list of int
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"""
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return self._metadata.frames
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@property
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def height(self):
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"""
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The height of each image in pixels.
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:rtype: int
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"""
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return self._metadata.height
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@property
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def width(self):
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"""
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The width of each image in pixels.
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:rtype: int
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"""
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return self._metadata.width
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def get_image(self, frame_number, field_of_view, channel_name, z_level):
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"""
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Attempts to return the image with the unique combination of given attributes. None will be returned if a match is not found.
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:type frame_number: int
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:param field_of_view: the label for the place in the XY-plane where this image was taken.
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:type field_of_view: int
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:param channel_name: the name of the color of this image
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:type channel_name: str
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:param z_level: the label for the location in the Z-plane where this image was taken.
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:type z_level: int
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:rtype: nd2reader.model.Image() or None
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"""
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return self._driver.get_image_by_attributes(frame_number, field_of_view, channel_name, z_level, self.height, self.width)
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def close(self):
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"""
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Closes the file handle to the image. This actually sometimes will prevent problems so it's good to do this or use Nd2 as a context manager.
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"""
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self._fh.close()
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