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from nd2reader.parser import Nd2Parser
import unittest
class MockNd2Parser(object):
def __init__(self, channels, fields_of_view, z_levels):
self.channels = channels
self.fields_of_view = fields_of_view
self.z_levels = z_levels
class TestNd2Parser(unittest.TestCase):
def test_calculate_field_of_view_simple(self):
""" With a single field of view, the field of view should always be the same number (0). """
nd2 = MockNd2Parser([''], [0], [0])
for frame_number in range(1000):
result = Nd2Parser._calculate_field_of_view(nd2, frame_number)
self.assertEqual(result, 0)
def test_calculate_field_of_view_two_channels(self):
nd2 = MockNd2Parser(['', 'GFP'], [0], [0])
for frame_number in range(1000):
result = Nd2Parser._calculate_field_of_view(nd2, frame_number)
self.assertEqual(result, 0)
def test_calculate_field_of_view_three_channels(self):
nd2 = MockNd2Parser(['', 'GFP', 'dsRed'], [0], [0])
for frame_number in range(1000):
result = Nd2Parser._calculate_field_of_view(nd2, frame_number)
self.assertEqual(result, 0)
def test_calculate_field_of_view_two_fovs(self):
nd2 = MockNd2Parser([''], [0, 1], [0])
for frame_number in range(1000):
result = Nd2Parser._calculate_field_of_view(nd2, frame_number)
self.assertEqual(result, frame_number % 2)
def test_calculate_field_of_view_two_fovs_two_zlevels(self):
nd2 = MockNd2Parser([''], [0, 1], [0, 1])
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 0), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 1), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 2), 1)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 3), 1)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 4), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 5), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 6), 1)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 7), 1)
def test_calculate_field_of_view_two_everything(self):
nd2 = MockNd2Parser(['', 'GFP'], [0, 1], [0, 1])
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 0), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 1), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 2), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 3), 0)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 4), 1)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 5), 1)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 6), 1)
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 7), 1)
def test_calculate_field_of_view_7c2f2z(self):
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0, 1], [0, 1])
for i in range(14):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0)
for i in range(14, 28):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1)
for i in range(28, 42):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0)
for i in range(42, 56):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1)
for i in range(56, 70):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0)
for i in range(70, 84):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1)
def test_calculate_field_of_view_2c3f5z(self):
""" All prime numbers to elucidate any errors that won't show up when numbers are multiples of each other """
nd2 = MockNd2Parser(['', 'GFP'], [0, 1, 2], [0, 1, 2, 3, 4])
for i in range(10):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0)
for i in range(10, 20):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1)
for i in range(20, 30):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 2)
for i in range(30, 40):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0)
for i in range(40, 50):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1)
for i in range(50, 60):
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 2)
def test_calculate_channel(self):
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0], [0])
for i in range(1000):
for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7):
self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel)
def test_calculate_channel_7c2fov1z(self):
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0, 1], [0])
for i in range(1000):
for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7):
self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel)
def test_calculate_channel_ludicrous_values(self):
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], list(range(31)), list(range(17)))
for i in range(1000):
for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7):
self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel)