66 driverszolfa-add_slices_loading
@ -1,152 +1 @@ | |||||
# -*- coding: utf-8 -*- | |||||
from nd2reader.model import Image, ImageSet | |||||
from nd2reader.parser import Nd2Parser | |||||
import six | |||||
class Nd2(Nd2Parser): | |||||
""" | |||||
Allows easy access to NIS Elements .nd2 image files. | |||||
""" | |||||
def __init__(self, filename): | |||||
super(Nd2, self).__init__(filename) | |||||
self._filename = filename | |||||
def __repr__(self): | |||||
return "\n".join(["<ND2 %s>" % self._filename, | |||||
"Created: %s" % self.absolute_start.strftime("%Y-%m-%d %H:%M:%S"), | |||||
"Image size: %sx%s (HxW)" % (self.height, self.width), | |||||
"Image cycles: %s" % len(self.time_indexes), | |||||
"Channels: %s" % ", ".join(["'%s'" % str(channel) for channel in self.channels]), | |||||
"Fields of View: %s" % len(self.fields_of_view), | |||||
"Z-Levels: %s" % len(self.z_levels) | |||||
]) | |||||
def __len__(self): | |||||
""" | |||||
This should be the total number of images in the ND2, but it may be inaccurate. If the ND2 contains a | |||||
different number of images in a cycle (i.e. there are "gap" images) it will be higher than reality. | |||||
:rtype: int | |||||
""" | |||||
return self._total_images_per_channel * len(self.channels) | |||||
def __getitem__(self, item): | |||||
""" | |||||
Allows slicing ND2s. | |||||
>>> nd2 = Nd2("my_images.nd2") | |||||
>>> image = nd2[16] # gets 17th frame | |||||
>>> for image in nd2[100:200]: # iterate over the 100th to 200th images | |||||
>>> do_something(image.data) | |||||
>>> for image in nd2[::-1]: # iterate backwards | |||||
>>> do_something(image.data) | |||||
>>> for image in nd2[37:422:17]: # do something super weird if you really want to | |||||
>>> do_something(image.data) | |||||
:type item: int or slice | |||||
:rtype: nd2reader.model.Image() or generator | |||||
""" | |||||
if isinstance(item, int): | |||||
try: | |||||
channel_offset = item % len(self.channels) | |||||
fov = self._calculate_field_of_view(item) | |||||
channel = self._calculate_channel(item) | |||||
z_level = self._calculate_z_level(item) | |||||
image_group_number = int(item / len(self.channels)) | |||||
frame_number = self._calculate_frame_number(image_group_number, fov, z_level) | |||||
timestamp, raw_image_data = self._get_raw_image_data(image_group_number, channel_offset) | |||||
image = Image(timestamp, frame_number, raw_image_data, fov, channel, z_level, self.height, self.width) | |||||
except (TypeError, ValueError): | |||||
return None | |||||
except KeyError: | |||||
raise IndexError("Invalid frame number.") | |||||
else: | |||||
return image | |||||
elif isinstance(item, slice): | |||||
return self._slice(item.start, item.stop, item.step) | |||||
raise IndexError | |||||
def _slice(self, start, stop, step): | |||||
""" | |||||
Allows for iteration over a selection of the entire dataset. | |||||
:type start: int | |||||
:type stop: int | |||||
:type step: int | |||||
:rtype: nd2reader.model.Image() or None | |||||
""" | |||||
start = start if start is not None else 0 | |||||
step = step if step is not None else 1 | |||||
stop = stop if stop is not None else len(self) | |||||
# This weird thing with the step allows you to iterate backwards over the images | |||||
for i in range(start, stop)[::step]: | |||||
yield self[i] | |||||
@property | |||||
def image_sets(self): | |||||
""" | |||||
Iterates over groups of related images. This is useful if your ND2 contains multiple fields of view. | |||||
A typical use case might be that you have, say, four areas of interest that you're monitoring, and every | |||||
minute you take a bright field and GFP image of each one. For each cycle, this method would produce four | |||||
ImageSet objects, each containing one bright field and one GFP image. | |||||
:return: model.ImageSet() | |||||
""" | |||||
for time_index in self.time_indexes: | |||||
image_set = ImageSet() | |||||
for fov in self.fields_of_view: | |||||
for channel_name in self.channels: | |||||
for z_level in self.z_levels: | |||||
image = self.get_image(time_index, fov, channel_name, z_level) | |||||
if image is not None: | |||||
image_set.add(image) | |||||
yield image_set | |||||
@property | |||||
def height(self): | |||||
""" | |||||
:return: height of each image, in pixels | |||||
:rtype: int | |||||
""" | |||||
return self.metadata[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiHeight')] | |||||
@property | |||||
def width(self): | |||||
""" | |||||
:return: width of each image, in pixels | |||||
:rtype: int | |||||
""" | |||||
return self.metadata[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiWidth')] | |||||
def get_image(self, frame_number, field_of_view, channel_name, z_level): | |||||
""" | |||||
Returns an Image if data exists for the given parameters, otherwise returns None. In general, you should avoid | |||||
using this method unless you're very familiar with the structure of ND2 files. | |||||
:type frame_number: int | |||||
:param field_of_view: the label for the place in the XY-plane where this image was taken. | |||||
:type field_of_view: int | |||||
:param channel_name: the name of the color of this image | |||||
:type channel_name: str | |||||
:param z_level: the label for the location in the Z-plane where this image was taken. | |||||
:type z_level: int | |||||
:rtype: nd2reader.model.Image() or None | |||||
""" | |||||
image_group_number = self._calculate_image_group_number(frame_number, field_of_view, z_level) | |||||
try: | |||||
timestamp, raw_image_data = self._get_raw_image_data(image_group_number, self._channel_offset[channel_name]) | |||||
image = Image(timestamp, frame_number, raw_image_data, field_of_view, channel_name, z_level, self.height, self.width) | |||||
except TypeError: | |||||
return None | |||||
else: | |||||
return image | |||||
from nd2reader.interface import Nd2 |
@ -0,0 +1,117 @@ | |||||
# -*- coding: utf-8 -*- | |||||
import array | |||||
import numpy as np | |||||
import struct | |||||
import six | |||||
from nd2reader.model.image import Image | |||||
class V3Driver(object): | |||||
CHUNK_HEADER = 0xabeceda | |||||
def __init__(self, metadata, label_map, file_handle): | |||||
self._metadata = metadata | |||||
self._label_map = label_map | |||||
self._file_handle = file_handle | |||||
def _calculate_field_of_view(self, frame_number): | |||||
images_per_cycle = len(self._metadata.z_levels) * len(self._metadata.channels) | |||||
return int((frame_number - (frame_number % images_per_cycle)) / images_per_cycle) % len(self._metadata.fields_of_view) | |||||
def _calculate_channel(self, frame_number): | |||||
return self._metadata.channels[frame_number % len(self._metadata.channels)] | |||||
def _calculate_z_level(self, frame_number): | |||||
return self._metadata.z_levels[int(((frame_number - (frame_number % len(self._metadata.channels))) / len(self._metadata.channels)) % len(self._metadata.z_levels))] | |||||
def _calculate_image_group_number(self, time_index, fov, z_level): | |||||
""" | |||||
Images are grouped together if they share the same time index, field of view, and z-level. | |||||
:type time_index: int | |||||
:type fov: int | |||||
:type z_level: int | |||||
:rtype: int | |||||
""" | |||||
return time_index * len(self._metadata.fields_of_view) * len(self._metadata.z_levels) + (fov * len(self._metadata.z_levels) + z_level) | |||||
def _calculate_frame_number(self, image_group_number, fov, z_level): | |||||
return (image_group_number - (fov * len(self._metadata.z_levels) + z_level)) / (len(self._metadata.fields_of_view) * len(self._metadata.z_levels)) | |||||
def get_image(self, index): | |||||
channel_offset = index % len(self._metadata.channels) | |||||
fov = self._calculate_field_of_view(index) | |||||
channel = self._calculate_channel(index) | |||||
z_level = self._calculate_z_level(index) | |||||
image_group_number = int(index / len(self._metadata.channels)) | |||||
frame_number = self._calculate_frame_number(image_group_number, fov, z_level) | |||||
timestamp, image = self._get_raw_image_data(image_group_number, channel_offset, self._metadata.height, self._metadata.width) | |||||
image.add_params(timestamp, frame_number, fov, channel, z_level) | |||||
return image | |||||
@property | |||||
def _channel_offset(self): | |||||
""" | |||||
Image data is interleaved for each image set. That is, if there are four images in a set, the first image | |||||
will consist of pixels 1, 5, 9, etc, the second will be pixels 2, 6, 10, and so forth. | |||||
:rtype: dict | |||||
""" | |||||
channel_offset = {} | |||||
for n, channel in enumerate(self._metadata.channels): | |||||
channel_offset[channel] = n | |||||
return channel_offset | |||||
def _get_raw_image_data(self, image_group_number, channel_offset, height, width): | |||||
""" | |||||
Reads the raw bytes and the timestamp of an image. | |||||
:param image_group_number: groups are made of images with the same time index, field of view and z-level. | |||||
:type image_group_number: int | |||||
:param channel_offset: the offset in the array where the bytes for this image are found. | |||||
:type channel_offset: int | |||||
:return: (int, array.array()) or None | |||||
""" | |||||
chunk = self._label_map[six.b("ImageDataSeq|%d!" % image_group_number)] | |||||
data = self._read_chunk(chunk) | |||||
# All images in the same image group share the same timestamp! So if you have complicated image data, | |||||
# your timestamps may not be entirely accurate. Practically speaking though, they'll only be off by a few | |||||
# seconds unless you're doing something super weird. | |||||
timestamp = struct.unpack("d", data[:8])[0] | |||||
image_group_data = array.array("H", data) | |||||
image_data_start = 4 + channel_offset | |||||
# The images for the various channels are interleaved within the same array. For example, the second image | |||||
# of a four image group will be composed of bytes 2, 6, 10, etc. If you understand why someone would design | |||||
# a data structure that way, please send the author of this library a message. | |||||
image_data = np.reshape(image_group_data[image_data_start::len(self._metadata.channels)], (height, width)) | |||||
# Skip images that are all zeros! This is important, since NIS Elements creates blank "gap" images if you | |||||
# don't have the same number of images each cycle. We discovered this because we only took GFP images every | |||||
# other cycle to reduce phototoxicity, but NIS Elements still allocated memory as if we were going to take | |||||
# them every cycle. | |||||
if np.any(image_data): | |||||
return timestamp, Image(image_data) | |||||
return None | |||||
def _read_chunk(self, chunk_location): | |||||
""" | |||||
Gets the data for a given chunk pointer | |||||
:rtype: bytes | |||||
""" | |||||
self._file_handle.seek(chunk_location) | |||||
# The chunk metadata is always 16 bytes long | |||||
chunk_metadata = self._file_handle.read(16) | |||||
header, relative_offset, data_length = struct.unpack("IIQ", chunk_metadata) | |||||
if header != V3Driver.CHUNK_HEADER: | |||||
raise ValueError("The ND2 file seems to be corrupted.") | |||||
# We start at the location of the chunk metadata, skip over the metadata, and then proceed to the | |||||
# start of the actual data field, which is at some arbitrary place after the metadata. | |||||
self._file_handle.seek(chunk_location + 16 + relative_offset) | |||||
return self._file_handle.read(data_length) |
@ -0,0 +1,2 @@ | |||||
class InvalidVersionError(Exception): | |||||
pass |
@ -0,0 +1,157 @@ | |||||
# -*- coding: utf-8 -*- | |||||
from nd2reader.model import ImageGroup | |||||
from nd2reader.parser import get_parser | |||||
from nd2reader.version import get_version | |||||
import warnings | |||||
class Nd2(object): | |||||
""" | |||||
Allows easy access to NIS Elements .nd2 image files. | |||||
""" | |||||
def __init__(self, filename): | |||||
major_version, minor_version = get_version(filename) | |||||
parser = get_parser(filename, major_version, minor_version) | |||||
self._driver = parser.driver | |||||
self._metadata = parser.metadata | |||||
self._filename = filename | |||||
def __repr__(self): | |||||
return "\n".join(["<ND2 %s>" % self._filename, | |||||
"Created: %s" % self.date, | |||||
"Image size: %sx%s (HxW)" % (self.height, self.width), | |||||
"Frames: %s" % len(self.frames), | |||||
"Channels: %s" % ", ".join(["'%s'" % str(channel) for channel in self.channels]), | |||||
"Fields of View: %s" % len(self.fields_of_view), | |||||
"Z-Levels: %s" % len(self.z_levels) | |||||
]) | |||||
def __len__(self): | |||||
""" | |||||
This should be the total number of images in the ND2, but it may be inaccurate. If the ND2 contains a | |||||
different number of images in a cycle (i.e. there are "gap" images) it will be higher than reality. | |||||
:rtype: int | |||||
""" | |||||
return self._driver.total_images_per_channel * len(self.channels) | |||||
def __getitem__(self, item): | |||||
""" | |||||
Allows slicing ND2s. | |||||
>>> nd2 = Nd2("my_images.nd2") | |||||
>>> image = nd2[16] # gets 17th frame | |||||
>>> for image in nd2[100:200]: # iterate over the 100th to 200th images | |||||
>>> do_something(image) | |||||
>>> for image in nd2[::-1]: # iterate backwards | |||||
>>> do_something(image) | |||||
>>> for image in nd2[37:422:17]: # do something super weird if you really want to | |||||
>>> do_something(image) | |||||
:type item: int or slice | |||||
:rtype: nd2reader.model.Image() or generator | |||||
""" | |||||
if isinstance(item, int): | |||||
return self._driver.get_image(item) | |||||
elif isinstance(item, slice): | |||||
return self._slice(item.start, item.stop, item.step) | |||||
raise IndexError | |||||
def _slice(self, start, stop, step): | |||||
""" | |||||
Allows for iteration over a selection of the entire dataset. | |||||
:type start: int | |||||
:type stop: int | |||||
:type step: int | |||||
:rtype: nd2reader.model.Image() | |||||
""" | |||||
start = start if start is not None else 0 | |||||
step = step if step is not None else 1 | |||||
stop = stop if stop is not None else len(self) | |||||
# This weird thing with the step allows you to iterate backwards over the images | |||||
for i in range(start, stop)[::step]: | |||||
yield self[i] | |||||
@property | |||||
def image_sets(self): | |||||
""" | |||||
Iterates over groups of related images. This is useful if your ND2 contains multiple fields of view. | |||||
A typical use case might be that you have, say, four areas of interest that you're monitoring, and every | |||||
minute you take a bright field and GFP image of each one. For each cycle, this method would produce four | |||||
ImageSet objects, each containing one bright field and one GFP image. | |||||
:return: model.ImageSet() | |||||
""" | |||||
warnings.warn("Nd2.image_sets will be removed from the nd2reader library in the near future.", DeprecationWarning) | |||||
for frame in self.frames: | |||||
image_group = ImageGroup() | |||||
for fov in self.fields_of_view: | |||||
for channel_name in self.channels: | |||||
for z_level in self.z_levels: | |||||
image = self.get_image(frame, fov, channel_name, z_level) | |||||
if image is not None: | |||||
image_group.add(image) | |||||
yield image_group | |||||
@property | |||||
def date(self): | |||||
return self._metadata.date | |||||
@property | |||||
def z_levels(self): | |||||
return self._metadata.z_levels | |||||
@property | |||||
def fields_of_view(self): | |||||
return self._metadata.fields_of_view | |||||
@property | |||||
def channels(self): | |||||
return self._metadata.channels | |||||
@property | |||||
def frames(self): | |||||
return self._metadata.frames | |||||
@property | |||||
def height(self): | |||||
""" | |||||
:return: height of each image, in pixels | |||||
:rtype: int | |||||
""" | |||||
return self._metadata.height | |||||
@property | |||||
def width(self): | |||||
""" | |||||
:return: width of each image, in pixels | |||||
:rtype: int | |||||
""" | |||||
return self._metadata.width | |||||
def get_image(self, frame_number, field_of_view, channel_name, z_level): | |||||
""" | |||||
Returns an Image if data exists for the given parameters, otherwise returns None. | |||||
:type frame_number: int | |||||
:param field_of_view: the label for the place in the XY-plane where this image was taken. | |||||
:type field_of_view: int | |||||
:param channel_name: the name of the color of this image | |||||
:type channel_name: str | |||||
:param z_level: the label for the location in the Z-plane where this image was taken. | |||||
:type z_level: int | |||||
:rtype: nd2reader.model.Image() | |||||
""" | |||||
return self._driver.get_image_by_attributes(frame_number, field_of_view, channel_name, z_level) |
@ -1,155 +1,2 @@ | |||||
# -*- coding: utf-8 -*- | |||||
import collections | |||||
import numpy as np | |||||
import logging | |||||
log = logging.getLogger(__name__) | |||||
class Image(object): | |||||
def __init__(self, timestamp, frame_number, raw_array, field_of_view, channel, z_level, height, width): | |||||
""" | |||||
A wrapper around the raw pixel data of an image. | |||||
:param timestamp: The frame number relative to the . | |||||
:type timestamp: int | |||||
:param timestamp: The number of milliseconds after the beginning of the acquisition that this image was taken. | |||||
:type timestamp: int | |||||
:param raw_array: The raw sequence of bytes that represents the image. | |||||
:type raw_array: array.array() | |||||
:param field_of_view: The label for the place in the XY-plane where this image was taken. | |||||
:type field_of_view: int | |||||
:param channel: The name of the color of this image | |||||
:type channel: str | |||||
:param z_level: The label for the location in the Z-plane where this image was taken. | |||||
:type z_level: int | |||||
:param height: The height of the image in pixels. | |||||
:type height: int | |||||
:param width: The width of the image in pixels. | |||||
:type width: int | |||||
""" | |||||
self._timestamp = timestamp | |||||
self._frame_number = int(frame_number) | |||||
self._raw_data = raw_array | |||||
self._field_of_view = field_of_view | |||||
self._channel = channel | |||||
self._z_level = z_level | |||||
self._height = height | |||||
self._width = width | |||||
self._data = None | |||||
def __repr__(self): | |||||
return "\n".join(["<ND2 Image>", | |||||
"%sx%s (HxW)" % (self._height, self._width), | |||||
"Timestamp: %s" % self.timestamp, | |||||
"Frame: %s" % self._frame_number, | |||||
"Field of View: %s" % self.field_of_view, | |||||
"Channel: %s" % self.channel, | |||||
"Z-Level: %s" % self.z_level, | |||||
]) | |||||
@property | |||||
def data(self): | |||||
""" | |||||
The actual image data. | |||||
:rtype np.array() | |||||
""" | |||||
if self._data is None: | |||||
# The data is just a 1-dimensional array originally. | |||||
# We convert it to a 2D image here. | |||||
self._data = np.reshape(self._raw_data, (self._height, self._width)) | |||||
return self._data | |||||
@property | |||||
def field_of_view(self): | |||||
""" | |||||
Which of the fixed locations this image was taken at. | |||||
:rtype int: | |||||
""" | |||||
return self._field_of_view | |||||
@property | |||||
def timestamp(self): | |||||
""" | |||||
The number of seconds after the beginning of the acquisition that the image was taken. Note that for a given | |||||
field of view and z-level offset, if you have images of multiple channels, they will all be given the same | |||||
timestamp. No, this doesn't make much sense. But that's how ND2s are structured, so if your experiment depends | |||||
on millisecond accuracy, you need to find an alternative imaging system. | |||||
:rtype float: | |||||
""" | |||||
return self._timestamp / 1000.0 | |||||
@property | |||||
def frame_number(self): | |||||
return self._frame_number | |||||
@property | |||||
def channel(self): | |||||
""" | |||||
The name of the filter used to acquire this image. These are user-supplied in NIS Elements. | |||||
:rtype str: | |||||
""" | |||||
return self._channel | |||||
@property | |||||
def z_level(self): | |||||
""" | |||||
The vertical offset of the image. These are simple integers starting from 0, where the 0 is the lowest | |||||
z-level and each subsequent level incremented by 1. | |||||
For example, if you acquired images at -3 µm, 0 µm, and +3 µm, your z-levels would be: | |||||
-3 µm: 0 | |||||
0 µm: 1 | |||||
+3 µm: 2 | |||||
:rtype int: | |||||
""" | |||||
return self._z_level | |||||
class ImageSet(object): | |||||
""" | |||||
A group of images that were taken at roughly the same time. | |||||
""" | |||||
def __init__(self): | |||||
self._images = collections.defaultdict(dict) | |||||
def __len__(self): | |||||
""" The number of images in the image set. """ | |||||
return sum([len(channel) for channel in self._images.values()]) | |||||
def __repr__(self): | |||||
return "\n".join(["<ND2 Image Set>", | |||||
"Image count: %s" % len(self)]) | |||||
def get(self, channel, z_level=0): | |||||
""" | |||||
Retrieve an image with a given channel and z-level. For most users, z_level will always be 0. | |||||
:type channel: str | |||||
:type z_level: int | |||||
""" | |||||
return self._images.get(channel).get(z_level) | |||||
def add(self, image): | |||||
""" | |||||
Stores an image. | |||||
:type image: nd2reader.model.Image() | |||||
""" | |||||
self._images[image.channel][image.z_level] = image | |||||
from nd2reader.model.image import Image | |||||
from nd2reader.model.group import ImageGroup |
@ -0,0 +1,37 @@ | |||||
import collections | |||||
class ImageGroup(object): | |||||
""" | |||||
A group of images that were taken at roughly the same time and in the same field of view. | |||||
""" | |||||
def __init__(self): | |||||
self._images = collections.defaultdict(dict) | |||||
def __len__(self): | |||||
""" The number of images in the image set. """ | |||||
return sum([len(channel) for channel in self._images.values()]) | |||||
def __repr__(self): | |||||
return "\n".join(["<ND2 Image Group>", | |||||
"Image count: %s" % len(self)]) | |||||
def get(self, channel, z_level=0): | |||||
""" | |||||
Retrieve an image with a given channel and z-level. For most users, z_level will always be 0. | |||||
:type channel: str | |||||
:type z_level: int | |||||
""" | |||||
return self._images.get(channel).get(z_level) | |||||
def add(self, image): | |||||
""" | |||||
Stores an image. | |||||
:type image: nd2reader.model.Image() | |||||
""" | |||||
self._images[image.channel][image.z_level] = image |
@ -0,0 +1,99 @@ | |||||
# -*- coding: utf-8 -*- | |||||
import numpy as np | |||||
class Image(np.ndarray): | |||||
def __new__(cls, array): | |||||
return np.asarray(array).view(cls) | |||||
def __init__(self, array): | |||||
self._timestamp = None | |||||
self._frame_number = None | |||||
self._field_of_view = None | |||||
self._channel = None | |||||
self._z_level = None | |||||
def add_params(self, timestamp, frame_number, field_of_view, channel, z_level): | |||||
""" | |||||
A wrapper around the raw pixel data of an image. | |||||
:param timestamp: The frame number relative to the . | |||||
:type timestamp: int | |||||
:param timestamp: The number of milliseconds after the beginning of the acquisition that this image was taken. | |||||
:type timestamp: int | |||||
:param field_of_view: The label for the place in the XY-plane where this image was taken. | |||||
:type field_of_view: int | |||||
:param channel: The name of the color of this image | |||||
:type channel: str | |||||
:param z_level: The label for the location in the Z-plane where this image was taken. | |||||
:type z_level: int | |||||
""" | |||||
self._timestamp = timestamp | |||||
self._frame_number = int(frame_number) | |||||
self._field_of_view = field_of_view | |||||
self._channel = channel | |||||
self._z_level = z_level | |||||
@property | |||||
def height(self): | |||||
return self.shape[1] | |||||
@property | |||||
def width(self): | |||||
return self.shape[0] | |||||
@property | |||||
def field_of_view(self): | |||||
""" | |||||
Which of the fixed locations this image was taken at. | |||||
:rtype int: | |||||
""" | |||||
return self._field_of_view | |||||
@property | |||||
def timestamp(self): | |||||
""" | |||||
The number of seconds after the beginning of the acquisition that the image was taken. Note that for a given | |||||
field of view and z-level offset, if you have images of multiple channels, they will all be given the same | |||||
timestamp. No, this doesn't make much sense. But that's how ND2s are structured, so if your experiment depends | |||||
on millisecond accuracy, you need to find an alternative imaging system. | |||||
:rtype float: | |||||
""" | |||||
return self._timestamp / 1000.0 | |||||
@property | |||||
def frame_number(self): | |||||
return self._frame_number | |||||
@property | |||||
def channel(self): | |||||
""" | |||||
The name of the filter used to acquire this image. These are user-supplied in NIS Elements. | |||||
:rtype str: | |||||
""" | |||||
return self._channel | |||||
@property | |||||
def z_level(self): | |||||
""" | |||||
The vertical offset of the image. These are simple integers starting from 0, where the 0 is the lowest | |||||
z-level and each subsequent level incremented by 1. | |||||
For example, if you acquired images at -3 µm, 0 µm, and +3 µm, your z-levels would be: | |||||
-3 µm: 0 | |||||
0 µm: 1 | |||||
+3 µm: 2 | |||||
:rtype int: | |||||
""" | |||||
return self._z_level |
@ -0,0 +1,72 @@ | |||||
class Metadata(object): | |||||
""" A simple container for ND2 metadata. """ | |||||
def __init__(self, height, width, channels, date, fields_of_view, frames, z_levels): | |||||
self._height = height | |||||
self._width = width | |||||
self._channels = channels | |||||
self._date = date | |||||
self._fields_of_view = fields_of_view | |||||
self._frames = frames | |||||
self._z_levels = z_levels | |||||
@property | |||||
def height(self): | |||||
return self._height | |||||
@property | |||||
def width(self): | |||||
return self._width | |||||
@property | |||||
def date(self): | |||||
""" | |||||
The date and time when acquisition began. | |||||
:rtype: datetime.datetime() | |||||
""" | |||||
return self._date | |||||
@property | |||||
def channels(self): | |||||
""" | |||||
These are labels created by the NIS Elements user. Typically they may a short description of the filter cube | |||||
used (e.g. "bright field", "GFP", etc.) | |||||
:rtype: list | |||||
""" | |||||
return self._channels | |||||
@property | |||||
def fields_of_view(self): | |||||
""" | |||||
The metadata contains information about fields of view, but it contains it even if some fields | |||||
of view were cropped. We can't find anything that states which fields of view are actually | |||||
in the image data, so we have to calculate it. There probably is something somewhere, since | |||||
NIS Elements can figure it out, but we haven't found it yet. | |||||
:rtype: list | |||||
""" | |||||
return self._fields_of_view | |||||
@property | |||||
def frames(self): | |||||
""" | |||||
The number of cycles. | |||||
:rtype: list | |||||
""" | |||||
return self._frames | |||||
@property | |||||
def z_levels(self): | |||||
""" | |||||
The different levels in the Z-plane. Just a sequence from 0 to n. | |||||
:rtype: list | |||||
""" | |||||
return self._z_levels |
@ -1,386 +0,0 @@ | |||||
# -*- coding: utf-8 -*- | |||||
import array | |||||
from datetime import datetime | |||||
import numpy as np | |||||
import re | |||||
import struct | |||||
import six | |||||
class Nd2Parser(object): | |||||
""" | |||||
Reads .nd2 files, provides an interface to the metadata, and generates numpy arrays from the image data. | |||||
You should not ever need to instantiate this class manually unless you're a developer. | |||||
""" | |||||
CHUNK_HEADER = 0xabeceda | |||||
CHUNK_MAP_START = six.b("ND2 FILEMAP SIGNATURE NAME 0001!") | |||||
CHUNK_MAP_END = six.b("ND2 CHUNK MAP SIGNATURE 0000001!") | |||||
def __init__(self, filename): | |||||
self._absolute_start = None | |||||
self._filename = filename | |||||
self._fh = None | |||||
self._channels = None | |||||
self._channel_count = None | |||||
self._chunk_map_start_location = None | |||||
self._cursor_position = 0 | |||||
self._dimension_text = None | |||||
self._fields_of_view = None | |||||
self._label_map = {} | |||||
self.metadata = {} | |||||
self._read_map() | |||||
self._time_indexes = None | |||||
self._parse_metadata() | |||||
self._z_levels = None | |||||
@property | |||||
def absolute_start(self): | |||||
""" | |||||
The date and time when acquisition began. | |||||
:rtype: datetime.datetime() | |||||
""" | |||||
if self._absolute_start is None: | |||||
for line in self.metadata[six.b('ImageTextInfo')][six.b('SLxImageTextInfo')].values(): | |||||
line = line.decode("utf8") | |||||
absolute_start_12 = None | |||||
absolute_start_24 = None | |||||
# ND2s seem to randomly switch between 12- and 24-hour representations. | |||||
try: | |||||
absolute_start_24 = datetime.strptime(line, "%m/%d/%Y %H:%M:%S") | |||||
except (TypeError, ValueError): | |||||
pass | |||||
try: | |||||
absolute_start_12 = datetime.strptime(line, "%m/%d/%Y %I:%M:%S %p") | |||||
except (TypeError, ValueError): | |||||
pass | |||||
if not absolute_start_12 and not absolute_start_24: | |||||
continue | |||||
return absolute_start_12 if absolute_start_12 else absolute_start_24 | |||||
raise ValueError("This ND2 has no recorded start time. This is probably a bug.") | |||||
return self._absolute_start | |||||
@property | |||||
def channels(self): | |||||
""" | |||||
These are labels created by the NIS Elements user. Typically they may a short description of the filter cube | |||||
used (e.g. "bright field", "GFP", etc.) | |||||
:rtype: list | |||||
""" | |||||
if not self._channels: | |||||
self._channels = [] | |||||
metadata = self.metadata[six.b('ImageMetadataSeq')][six.b('SLxPictureMetadata')][six.b('sPicturePlanes')] | |||||
try: | |||||
validity = self.metadata[six.b('ImageMetadata')][six.b('SLxExperiment')][six.b('ppNextLevelEx')][six.b('')][0][six.b('ppNextLevelEx')][six.b('')][0][six.b('pItemValid')] | |||||
except KeyError: | |||||
# If none of the channels have been deleted, there is no validity list, so we just make one | |||||
validity = [True for _ in metadata] | |||||
# Channel information is contained in dictionaries with the keys a0, a1...an where the number | |||||
# indicates the order in which the channel is stored. So by sorting the dicts alphabetically | |||||
# we get the correct order. | |||||
for (label, chan), valid in zip(sorted(metadata[six.b('sPlaneNew')].items()), validity): | |||||
if not valid: | |||||
continue | |||||
self._channels.append(chan[six.b('sDescription')].decode("utf8")) | |||||
return self._channels | |||||
@property | |||||
def fields_of_view(self): | |||||
""" | |||||
The metadata contains information about fields of view, but it contains it even if some fields | |||||
of view were cropped. We can't find anything that states which fields of view are actually | |||||
in the image data, so we have to calculate it. There probably is something somewhere, since | |||||
NIS Elements can figure it out, but we haven't found it yet. | |||||
:rtype: list | |||||
""" | |||||
if self._fields_of_view is None: | |||||
self._fields_of_view = self._parse_dimension_text(r""".*?XY\((\d+)\).*?""") | |||||
return self._fields_of_view | |||||
@property | |||||
def time_indexes(self): | |||||
""" | |||||
The number of cycles. | |||||
:rtype: list | |||||
""" | |||||
if self._time_indexes is None: | |||||
self._time_indexes = self._parse_dimension_text(r""".*?T'\((\d+)\).*?""") | |||||
return self._time_indexes | |||||
@property | |||||
def z_levels(self): | |||||
""" | |||||
The different levels in the Z-plane. Just a sequence from 0 to n. | |||||
:rtype: list | |||||
""" | |||||
if self._z_levels is None: | |||||
self._z_levels = self._parse_dimension_text(r""".*?Z\((\d+)\).*?""") | |||||
return self._z_levels | |||||
def _calculate_field_of_view(self, frame_number): | |||||
images_per_cycle = len(self.z_levels) * len(self.channels) | |||||
return int((frame_number - (frame_number % images_per_cycle)) / images_per_cycle) % len(self.fields_of_view) | |||||
def _calculate_channel(self, frame_number): | |||||
return self.channels[frame_number % len(self.channels)] | |||||
def _calculate_z_level(self, frame_number): | |||||
return self.z_levels[int(((frame_number - (frame_number % len(self.channels))) / len(self.channels)) % len(self.z_levels))] | |||||
@property | |||||
def _file_handle(self): | |||||
if self._fh is None: | |||||
self._fh = open(self._filename, "rb") | |||||
return self._fh | |||||
def _get_raw_image_data(self, image_group_number, channel_offset): | |||||
""" | |||||
Reads the raw bytes and the timestamp of an image. | |||||
:param image_group_number: groups are made of images with the same time index, field of view and z-level. | |||||
:type image_group_number: int | |||||
:param channel_offset: the offset in the array where the bytes for this image are found. | |||||
:type channel_offset: int | |||||
:return: (int, array.array()) or None | |||||
""" | |||||
chunk = self._label_map[six.b("ImageDataSeq|%d!" % image_group_number)] | |||||
data = self._read_chunk(chunk) | |||||
# All images in the same image group share the same timestamp! So if you have complicated image data, | |||||
# your timestamps may not be entirely accurate. Practically speaking though, they'll only be off by a few | |||||
# seconds unless you're doing something super weird. | |||||
timestamp = struct.unpack("d", data[:8])[0] | |||||
image_group_data = array.array("H", data) | |||||
image_data_start = 4 + channel_offset | |||||
# The images for the various channels are interleaved within the same array. For example, the second image | |||||
# of a four image group will be composed of bytes 2, 6, 10, etc. If you understand why someone would design | |||||
# a data structure that way, please send the author of this library a message. | |||||
image_data = image_group_data[image_data_start::len(self.channels)] | |||||
# Skip images that are all zeros! This is important, since NIS Elements creates blank "gap" images if you | |||||
# don't have the same number of images each cycle. We discovered this because we only took GFP images every | |||||
# other cycle to reduce phototoxicity, but NIS Elements still allocated memory as if we were going to take | |||||
# them every cycle. | |||||
if np.any(image_data): | |||||
return timestamp, image_data | |||||
return None | |||||
@property | |||||
def _dimensions(self): | |||||
""" | |||||
While there are metadata values that represent a lot of what we want to capture, they seem to be unreliable. | |||||
Sometimes certain elements don't exist, or change their data type randomly. However, the human-readable text | |||||
is always there and in the same exact format, so we just parse that instead. | |||||
:rtype: str | |||||
""" | |||||
if self._dimension_text is None: | |||||
for line in self.metadata[six.b('ImageTextInfo')][six.b('SLxImageTextInfo')].values(): | |||||
if six.b("Dimensions:") in line: | |||||
metadata = line | |||||
break | |||||
else: | |||||
raise ValueError("Could not parse metadata dimensions!") | |||||
for line in metadata.split(six.b("\r\n")): | |||||
if line.startswith(six.b("Dimensions:")): | |||||
self._dimension_text = line | |||||
break | |||||
else: | |||||
raise ValueError("Could not parse metadata dimensions!") | |||||
return self._dimension_text | |||||
def _calculate_image_group_number(self, time_index, fov, z_level): | |||||
""" | |||||
Images are grouped together if they share the same time index, field of view, and z-level. | |||||
:type time_index: int | |||||
:type fov: int | |||||
:type z_level: int | |||||
:rtype: int | |||||
""" | |||||
return time_index * len(self.fields_of_view) * len(self.z_levels) + (fov * len(self.z_levels) + z_level) | |||||
def _calculate_frame_number(self, image_group_number, fov, z_level): | |||||
return (image_group_number - (fov * len(self.z_levels) + z_level)) / (len(self.fields_of_view) * len(self.z_levels)) | |||||
@property | |||||
def _channel_offset(self): | |||||
""" | |||||
Image data is interleaved for each image set. That is, if there are four images in a set, the first image | |||||
will consist of pixels 1, 5, 9, etc, the second will be pixels 2, 6, 10, and so forth. | |||||
:rtype: dict | |||||
""" | |||||
channel_offset = {} | |||||
for n, channel in enumerate(self._channels): | |||||
channel_offset[channel] = n | |||||
return channel_offset | |||||
def _parse_dimension_text(self, pattern): | |||||
try: | |||||
count = int(re.match(pattern, self._dimensions).group(1)) | |||||
except AttributeError: | |||||
return [0] | |||||
except TypeError: | |||||
match = re.match(pattern, self._dimensions.decode("utf8")) | |||||
if not match: | |||||
return [0] | |||||
return list(range(int(match.group(1)))) | |||||
else: | |||||
return list(range(count)) | |||||
@property | |||||
def _total_images_per_channel(self): | |||||
""" | |||||
The total number of images per channel. Warning: this may be inaccurate as it includes "gap" images. | |||||
:rtype: int | |||||
""" | |||||
return self.metadata[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiSequenceCount')] | |||||
def _parse_metadata(self): | |||||
""" | |||||
Reads all metadata. | |||||
""" | |||||
for label in self._label_map.keys(): | |||||
if label.endswith(six.b("LV!")) or six.b("LV|") in label: | |||||
data = self._read_chunk(self._label_map[label]) | |||||
stop = label.index(six.b("LV")) | |||||
self.metadata[label[:stop]] = self._read_metadata(data, 1) | |||||
def _read_map(self): | |||||
""" | |||||
Every label ends with an exclamation point, however, we can't directly search for those to find all the labels | |||||
as some of the bytes contain the value 33, which is the ASCII code for "!". So we iteratively find each label, | |||||
grab the subsequent data (always 16 bytes long), advance to the next label and repeat. | |||||
""" | |||||
self._file_handle.seek(-8, 2) | |||||
chunk_map_start_location = struct.unpack("Q", self._file_handle.read(8))[0] | |||||
self._file_handle.seek(chunk_map_start_location) | |||||
raw_text = self._file_handle.read(-1) | |||||
label_start = raw_text.index(Nd2Parser.CHUNK_MAP_START) + 32 | |||||
while True: | |||||
data_start = raw_text.index(six.b("!"), label_start) + 1 | |||||
key = raw_text[label_start: data_start] | |||||
location, length = struct.unpack("QQ", raw_text[data_start: data_start + 16]) | |||||
if key == Nd2Parser.CHUNK_MAP_END: | |||||
# We've reached the end of the chunk map | |||||
break | |||||
self._label_map[key] = location | |||||
label_start = data_start + 16 | |||||
def _read_chunk(self, chunk_location): | |||||
""" | |||||
Gets the data for a given chunk pointer | |||||
""" | |||||
self._file_handle.seek(chunk_location) | |||||
# The chunk metadata is always 16 bytes long | |||||
chunk_metadata = self._file_handle.read(16) | |||||
header, relative_offset, data_length = struct.unpack("IIQ", chunk_metadata) | |||||
if header != Nd2Parser.CHUNK_HEADER: | |||||
raise ValueError("The ND2 file seems to be corrupted.") | |||||
# We start at the location of the chunk metadata, skip over the metadata, and then proceed to the | |||||
# start of the actual data field, which is at some arbitrary place after the metadata. | |||||
self._file_handle.seek(chunk_location + 16 + relative_offset) | |||||
return self._file_handle.read(data_length) | |||||
def _parse_unsigned_char(self, data): | |||||
return struct.unpack("B", data.read(1))[0] | |||||
def _parse_unsigned_int(self, data): | |||||
return struct.unpack("I", data.read(4))[0] | |||||
def _parse_unsigned_long(self, data): | |||||
return struct.unpack("Q", data.read(8))[0] | |||||
def _parse_double(self, data): | |||||
return struct.unpack("d", data.read(8))[0] | |||||
def _parse_string(self, data): | |||||
value = data.read(2) | |||||
while not value.endswith(six.b("\x00\x00")): | |||||
# the string ends at the first instance of \x00\x00 | |||||
value += data.read(2) | |||||
return value.decode("utf16")[:-1].encode("utf8") | |||||
def _parse_char_array(self, data): | |||||
array_length = struct.unpack("Q", data.read(8))[0] | |||||
return array.array("B", data.read(array_length)) | |||||
def _parse_metadata_item(self, data): | |||||
""" | |||||
Reads hierarchical data, analogous to a Python dict. | |||||
""" | |||||
new_count, length = struct.unpack("<IQ", data.read(12)) | |||||
length -= data.tell() - self._cursor_position | |||||
next_data_length = data.read(length) | |||||
value = self._read_metadata(next_data_length, new_count) | |||||
# Skip some offsets | |||||
data.read(new_count * 8) | |||||
return value | |||||
def _get_value(self, data, data_type): | |||||
""" | |||||
ND2s use various codes to indicate different data types, which we translate here. | |||||
""" | |||||
parser = {1: self._parse_unsigned_char, | |||||
2: self._parse_unsigned_int, | |||||
3: self._parse_unsigned_int, | |||||
5: self._parse_unsigned_long, | |||||
6: self._parse_double, | |||||
8: self._parse_string, | |||||
9: self._parse_char_array, | |||||
11: self._parse_metadata_item} | |||||
return parser[data_type](data) | |||||
def _read_metadata(self, data, count): | |||||
""" | |||||
Iterates over each element some section of the metadata and parses it. | |||||
""" | |||||
data = six.BytesIO(data) | |||||
metadata = {} | |||||
for _ in range(count): | |||||
self._cursor_position = data.tell() | |||||
header = data.read(2) | |||||
if not header: | |||||
# We've reached the end of some hierarchy of data | |||||
break | |||||
if six.PY3: | |||||
header = header.decode("utf8") | |||||
data_type, name_length = map(ord, header) | |||||
name = data.read(name_length * 2).decode("utf16")[:-1].encode("utf8") | |||||
value = self._get_value(data, data_type) | |||||
if name not in metadata.keys(): | |||||
metadata[name] = value | |||||
else: | |||||
if not isinstance(metadata[name], list): | |||||
# We have encountered this key exactly once before. Since we're seeing it again, we know we | |||||
# need to convert it to a list before proceeding. | |||||
metadata[name] = [metadata[name]] | |||||
# We've encountered this key before so we're guaranteed to be dealing with a list. Thus we append | |||||
# the value to the already-existing list. | |||||
metadata[name].append(value) | |||||
return metadata |
@ -0,0 +1 @@ | |||||
from . parser import get_parser |
@ -0,0 +1,11 @@ | |||||
from abc import abstractproperty | |||||
class BaseParser(object): | |||||
@abstractproperty | |||||
def metadata(self): | |||||
raise NotImplementedError | |||||
@abstractproperty | |||||
def driver(self): | |||||
raise NotImplementedError |
@ -0,0 +1,10 @@ | |||||
from nd2reader.parser.v3 import V3Parser | |||||
from nd2reader.exc import InvalidVersionError | |||||
def get_parser(filename, major_version, minor_version): | |||||
parsers = {(3, None): V3Parser} | |||||
parser = parsers.get((major_version, minor_version)) or parsers.get((major_version, None)) | |||||
if not parser: | |||||
raise InvalidVersionError("No parser is available for that version.") | |||||
return parser(filename) |
@ -0,0 +1,307 @@ | |||||
# -*- coding: utf-8 -*- | |||||
import array | |||||
from datetime import datetime | |||||
from nd2reader.model.metadata import Metadata | |||||
from nd2reader.parser.base import BaseParser | |||||
from nd2reader.driver.v3 import V3Driver | |||||
import re | |||||
import six | |||||
import struct | |||||
class V3Parser(BaseParser): | |||||
""" Parses ND2 files and creates a Metadata and ImageReader object. """ | |||||
CHUNK_HEADER = 0xabeceda | |||||
CHUNK_MAP_START = six.b("ND2 FILEMAP SIGNATURE NAME 0001!") | |||||
CHUNK_MAP_END = six.b("ND2 CHUNK MAP SIGNATURE 0000001!") | |||||
def __init__(self, filename): | |||||
self._filename = filename | |||||
self._fh = None | |||||
self._metadata = None | |||||
self._label_map = None | |||||
@property | |||||
def metadata(self): | |||||
if not self._metadata: | |||||
self._parse_metadata() | |||||
return self._metadata | |||||
@property | |||||
def driver(self): | |||||
return V3Driver(self.metadata, self._label_map, self._get_file_handle()) | |||||
def _parse_metadata(self): | |||||
""" | |||||
Reads all metadata. | |||||
""" | |||||
metadata_dict = {} | |||||
self._label_map = self._build_label_map() | |||||
for label in self._label_map.keys(): | |||||
if label.endswith(six.b("LV!")) or six.b("LV|") in label: | |||||
data = self._read_chunk(self._label_map[label]) | |||||
stop = label.index(six.b("LV")) | |||||
metadata_dict[label[:stop]] = self._read_metadata(data, 1) | |||||
height = metadata_dict[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiHeight')] | |||||
width = metadata_dict[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiWidth')] | |||||
channels = self._parse_channels(metadata_dict) | |||||
date = self._parse_date(metadata_dict) | |||||
fields_of_view = self._parse_fields_of_view(metadata_dict) | |||||
frames = self._parse_frames(metadata_dict) | |||||
z_levels = self._parse_z_levels(metadata_dict) | |||||
self._metadata = Metadata(height, width, channels, date, fields_of_view, frames, z_levels) | |||||
def _parse_date(self, metadata_dict): | |||||
""" | |||||
The date and time when acquisition began. | |||||
:rtype: datetime.datetime() | |||||
""" | |||||
for line in metadata_dict[six.b('ImageTextInfo')][six.b('SLxImageTextInfo')].values(): | |||||
line = line.decode("utf8") | |||||
absolute_start_12 = None | |||||
absolute_start_24 = None | |||||
# ND2s seem to randomly switch between 12- and 24-hour representations. | |||||
try: | |||||
absolute_start_24 = datetime.strptime(line, "%m/%d/%Y %H:%M:%S") | |||||
except (TypeError, ValueError): | |||||
pass | |||||
try: | |||||
absolute_start_12 = datetime.strptime(line, "%m/%d/%Y %I:%M:%S %p") | |||||
except (TypeError, ValueError): | |||||
pass | |||||
if not absolute_start_12 and not absolute_start_24: | |||||
continue | |||||
return absolute_start_12 if absolute_start_12 else absolute_start_24 | |||||
raise ValueError("This ND2 has no recorded start time. This is probably a bug.") | |||||
def _parse_channels(self, metadata_dict): | |||||
""" | |||||
These are labels created by the NIS Elements user. Typically they may a short description of the filter cube | |||||
used (e.g. "bright field", "GFP", etc.) | |||||
:rtype: list | |||||
""" | |||||
channels = [] | |||||
metadata = metadata_dict[six.b('ImageMetadataSeq')][six.b('SLxPictureMetadata')][six.b('sPicturePlanes')] | |||||
try: | |||||
validity = metadata_dict[six.b('ImageMetadata')][six.b('SLxExperiment')][six.b('ppNextLevelEx')][six.b('')][0][six.b('ppNextLevelEx')][six.b('')][0][six.b('pItemValid')] | |||||
except KeyError: | |||||
# If none of the channels have been deleted, there is no validity list, so we just make one | |||||
validity = [True for _ in metadata] | |||||
# Channel information is contained in dictionaries with the keys a0, a1...an where the number | |||||
# indicates the order in which the channel is stored. So by sorting the dicts alphabetically | |||||
# we get the correct order. | |||||
for (label, chan), valid in zip(sorted(metadata[six.b('sPlaneNew')].items()), validity): | |||||
if not valid: | |||||
continue | |||||
channels.append(chan[six.b('sDescription')].decode("utf8")) | |||||
return channels | |||||
def _parse_fields_of_view(self, metadata_dict): | |||||
""" | |||||
The metadata contains information about fields of view, but it contains it even if some fields | |||||
of view were cropped. We can't find anything that states which fields of view are actually | |||||
in the image data, so we have to calculate it. There probably is something somewhere, since | |||||
NIS Elements can figure it out, but we haven't found it yet. | |||||
:rtype: list | |||||
""" | |||||
return self._parse_dimension(r""".*?XY\((\d+)\).*?""", metadata_dict) | |||||
def _parse_frames(self, metadata_dict): | |||||
""" | |||||
The number of cycles. | |||||
:rtype: list | |||||
""" | |||||
return self._parse_dimension(r""".*?T'\((\d+)\).*?""", metadata_dict) | |||||
def _parse_z_levels(self, metadata_dict): | |||||
""" | |||||
The different levels in the Z-plane. Just a sequence from 0 to n. | |||||
:rtype: list | |||||
""" | |||||
return self._parse_dimension(r""".*?Z\((\d+)\).*?""", metadata_dict) | |||||
def _get_file_handle(self): | |||||
if self._fh is None: | |||||
self._fh = open(self._filename, "rb") | |||||
return self._fh | |||||
def _parse_dimension_text(self, metadata_dict): | |||||
""" | |||||
While there are metadata values that represent a lot of what we want to capture, they seem to be unreliable. | |||||
Sometimes certain elements don't exist, or change their data type randomly. However, the human-readable text | |||||
is always there and in the same exact format, so we just parse that instead. | |||||
:rtype: str | |||||
""" | |||||
for line in metadata_dict[six.b('ImageTextInfo')][six.b('SLxImageTextInfo')].values(): | |||||
if six.b("Dimensions:") in line: | |||||
metadata = line | |||||
break | |||||
else: | |||||
raise ValueError("Could not parse metadata dimensions!") | |||||
for line in metadata.split(six.b("\r\n")): | |||||
if line.startswith(six.b("Dimensions:")): | |||||
dimension_text = line | |||||
break | |||||
else: | |||||
raise ValueError("Could not parse metadata dimensions!") | |||||
return dimension_text | |||||
def _parse_dimension(self, pattern, metadata_dict): | |||||
dimension_text = self._parse_dimension_text(metadata_dict) | |||||
if six.PY3: | |||||
dimension_text = dimension_text.decode("utf8") | |||||
match = re.match(pattern, dimension_text) | |||||
if not match: | |||||
return [0] | |||||
count = int(match.group(1)) | |||||
return list(range(count)) | |||||
def _parse_total_images_per_channel(self, metadata_dict): | |||||
""" | |||||
The total number of images per channel. Warning: this may be inaccurate as it includes "gap" images. | |||||
:rtype: int | |||||
""" | |||||
return metadata_dict[six.b('ImageAttributes')][six.b('SLxImageAttributes')][six.b('uiSequenceCount')] | |||||
def _build_label_map(self): | |||||
""" | |||||
Every label ends with an exclamation point, however, we can't directly search for those to find all the labels | |||||
as some of the bytes contain the value 33, which is the ASCII code for "!". So we iteratively find each label, | |||||
grab the subsequent data (always 16 bytes long), advance to the next label and repeat. | |||||
:rtype: dict | |||||
""" | |||||
label_map = {} | |||||
self._get_file_handle().seek(-8, 2) | |||||
chunk_map_start_location = struct.unpack("Q", self._get_file_handle().read(8))[0] | |||||
self._get_file_handle().seek(chunk_map_start_location) | |||||
raw_text = self._get_file_handle().read(-1) | |||||
label_start = raw_text.index(V3Parser.CHUNK_MAP_START) + 32 | |||||
while True: | |||||
data_start = raw_text.index(six.b("!"), label_start) + 1 | |||||
key = raw_text[label_start: data_start] | |||||
location, length = struct.unpack("QQ", raw_text[data_start: data_start + 16]) | |||||
if key == V3Parser.CHUNK_MAP_END: | |||||
# We've reached the end of the chunk map | |||||
break | |||||
label_map[key] = location | |||||
label_start = data_start + 16 | |||||
return label_map | |||||
def _read_chunk(self, chunk_location): | |||||
""" | |||||
Gets the data for a given chunk pointer | |||||
:rtype: bytes | |||||
""" | |||||
self._get_file_handle().seek(chunk_location) | |||||
# The chunk metadata is always 16 bytes long | |||||
chunk_metadata = self._get_file_handle().read(16) | |||||
header, relative_offset, data_length = struct.unpack("IIQ", chunk_metadata) | |||||
if header != V3Parser.CHUNK_HEADER: | |||||
raise ValueError("The ND2 file seems to be corrupted.") | |||||
# We start at the location of the chunk metadata, skip over the metadata, and then proceed to the | |||||
# start of the actual data field, which is at some arbitrary place after the metadata. | |||||
self._get_file_handle().seek(chunk_location + 16 + relative_offset) | |||||
return self._get_file_handle().read(data_length) | |||||
def _parse_unsigned_char(self, data): | |||||
return struct.unpack("B", data.read(1))[0] | |||||
def _parse_unsigned_int(self, data): | |||||
return struct.unpack("I", data.read(4))[0] | |||||
def _parse_unsigned_long(self, data): | |||||
return struct.unpack("Q", data.read(8))[0] | |||||
def _parse_double(self, data): | |||||
return struct.unpack("d", data.read(8))[0] | |||||
def _parse_string(self, data): | |||||
value = data.read(2) | |||||
while not value.endswith(six.b("\x00\x00")): | |||||
# the string ends at the first instance of \x00\x00 | |||||
value += data.read(2) | |||||
return value.decode("utf16")[:-1].encode("utf8") | |||||
def _parse_char_array(self, data): | |||||
array_length = struct.unpack("Q", data.read(8))[0] | |||||
return array.array("B", data.read(array_length)) | |||||
def _parse_metadata_item(self, data): | |||||
""" | |||||
Reads hierarchical data, analogous to a Python dict. | |||||
""" | |||||
new_count, length = struct.unpack("<IQ", data.read(12)) | |||||
length -= data.tell() - self._cursor_position | |||||
next_data_length = data.read(length) | |||||
value = self._read_metadata(next_data_length, new_count) | |||||
# Skip some offsets | |||||
data.read(new_count * 8) | |||||
return value | |||||
def _get_value(self, data, data_type): | |||||
""" | |||||
ND2s use various codes to indicate different data types, which we translate here. | |||||
""" | |||||
parser = {1: self._parse_unsigned_char, | |||||
2: self._parse_unsigned_int, | |||||
3: self._parse_unsigned_int, | |||||
5: self._parse_unsigned_long, | |||||
6: self._parse_double, | |||||
8: self._parse_string, | |||||
9: self._parse_char_array, | |||||
11: self._parse_metadata_item} | |||||
return parser[data_type](data) | |||||
def _read_metadata(self, data, count): | |||||
""" | |||||
Iterates over each element some section of the metadata and parses it. | |||||
""" | |||||
data = six.BytesIO(data) | |||||
metadata = {} | |||||
for _ in range(count): | |||||
self._cursor_position = data.tell() | |||||
header = data.read(2) | |||||
if not header: | |||||
# We've reached the end of some hierarchy of data | |||||
break | |||||
if six.PY3: | |||||
header = header.decode("utf8") | |||||
data_type, name_length = map(ord, header) | |||||
name = data.read(name_length * 2).decode("utf16")[:-1].encode("utf8") | |||||
value = self._get_value(data, data_type) | |||||
if name not in metadata.keys(): | |||||
metadata[name] = value | |||||
else: | |||||
if not isinstance(metadata[name], list): | |||||
# We have encountered this key exactly once before. Since we're seeing it again, we know we | |||||
# need to convert it to a list before proceeding. | |||||
metadata[name] = [metadata[name]] | |||||
# We've encountered this key before so we're guaranteed to be dealing with a list. Thus we append | |||||
# the value to the already-existing list. | |||||
metadata[name].append(value) | |||||
return metadata |
@ -0,0 +1,33 @@ | |||||
import re | |||||
from nd2reader.exc import InvalidVersionError | |||||
def get_version(filename): | |||||
""" | |||||
Determines what version the ND2 is. | |||||
:param filename: the path (absolute or relative) to the ND2 | |||||
:type filename: str | |||||
""" | |||||
with open(filename, 'rb') as f: | |||||
# the first 16 bytes seem to have no meaning, so we skip them | |||||
f.seek(16) | |||||
# the next 38 bytes contain the string that we want to parse. Unlike most of the ND2, this is in UTF-8 | |||||
data = f.read(38).decode("utf8") | |||||
return parse_version(data) | |||||
def parse_version(data): | |||||
""" | |||||
Parses a string with the version data in it. | |||||
:param data: the 19th through 54th byte of the ND2, representing the version | |||||
:type data: unicode | |||||
""" | |||||
match = re.search(r"""^ND2 FILE SIGNATURE CHUNK NAME01!Ver(?P<major>\d)\.(?P<minor>\d)$""", data) | |||||
if match: | |||||
# We haven't seen a lot of ND2s but the ones we have seen conform to this | |||||
return int(match.group('major')), int(match.group('minor')) | |||||
raise InvalidVersionError("The version of the ND2 you specified is not supported.") |
@ -1,183 +1,183 @@ | |||||
from nd2reader.parser import Nd2Parser | |||||
import unittest | |||||
class MockNd2Parser(object): | |||||
def __init__(self, channels, fields_of_view, z_levels): | |||||
self.channels = channels | |||||
self.fields_of_view = fields_of_view | |||||
self.z_levels = z_levels | |||||
class TestNd2Parser(unittest.TestCase): | |||||
def test_calculate_field_of_view_simple(self): | |||||
""" With a single field of view, the field of view should always be the same number (0). """ | |||||
nd2 = MockNd2Parser([''], [0], [0]) | |||||
for frame_number in range(1000): | |||||
result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
self.assertEqual(result, 0) | |||||
def test_calculate_field_of_view_two_channels(self): | |||||
nd2 = MockNd2Parser(['', 'GFP'], [0], [0]) | |||||
for frame_number in range(1000): | |||||
result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
self.assertEqual(result, 0) | |||||
def test_calculate_field_of_view_three_channels(self): | |||||
nd2 = MockNd2Parser(['', 'GFP', 'dsRed'], [0], [0]) | |||||
for frame_number in range(1000): | |||||
result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
self.assertEqual(result, 0) | |||||
def test_calculate_field_of_view_two_fovs(self): | |||||
nd2 = MockNd2Parser([''], [0, 1], [0]) | |||||
for frame_number in range(1000): | |||||
result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
self.assertEqual(result, frame_number % 2) | |||||
def test_calculate_field_of_view_two_fovs_two_zlevels(self): | |||||
nd2 = MockNd2Parser([''], [0, 1], [0, 1]) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 0), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 1), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 2), 1) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 3), 1) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 4), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 5), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 6), 1) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 7), 1) | |||||
def test_calculate_field_of_view_two_everything(self): | |||||
nd2 = MockNd2Parser(['', 'GFP'], [0, 1], [0, 1]) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 0), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 1), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 2), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 3), 0) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 4), 1) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 5), 1) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 6), 1) | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 7), 1) | |||||
def test_calculate_field_of_view_7c2f2z(self): | |||||
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0, 1], [0, 1]) | |||||
for i in range(14): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
for i in range(14, 28): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
for i in range(28, 42): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
for i in range(42, 56): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
for i in range(56, 70): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
for i in range(70, 84): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
def test_calculate_field_of_view_2c3f5z(self): | |||||
""" All prime numbers to elucidate any errors that won't show up when numbers are multiples of each other """ | |||||
nd2 = MockNd2Parser(['', 'GFP'], [0, 1, 2], [0, 1, 2, 3, 4]) | |||||
for i in range(10): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
for i in range(10, 20): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
for i in range(20, 30): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 2) | |||||
for i in range(30, 40): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
for i in range(40, 50): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
for i in range(50, 60): | |||||
self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 2) | |||||
def test_calculate_channel_simple(self): | |||||
nd2 = MockNd2Parser(['GFP'], [0], [0]) | |||||
for i in range(1000): | |||||
self.assertEqual(Nd2Parser._calculate_channel(nd2, i), 'GFP') | |||||
def test_calculate_channel(self): | |||||
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0], [0]) | |||||
for i in range(1000): | |||||
for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7): | |||||
self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel) | |||||
def test_calculate_channel_7c2fov1z(self): | |||||
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0, 1], [0]) | |||||
for i in range(1000): | |||||
for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7): | |||||
self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel) | |||||
def test_calculate_channel_ludicrous_values(self): | |||||
nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], list(range(31)), list(range(17))) | |||||
for i in range(10000): | |||||
for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7): | |||||
self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel) | |||||
def test_calculate_z_level(self): | |||||
nd2 = MockNd2Parser([''], [0], [0]) | |||||
for frame_number in range(1000): | |||||
result = Nd2Parser._calculate_z_level(nd2, frame_number) | |||||
self.assertEqual(result, 0) | |||||
def test_calculate_z_level_1c1f2z(self): | |||||
nd2 = MockNd2Parser([''], [0], [0, 1]) | |||||
for frame_number in range(1000): | |||||
result = Nd2Parser._calculate_z_level(nd2, frame_number) | |||||
self.assertEqual(result, frame_number % 2) | |||||
def test_calculate_z_level_31c17f1z(self): | |||||
nd2 = MockNd2Parser(list(range(31)), list(range(17)), [0]) | |||||
for frame_number in range(1000): | |||||
result = Nd2Parser._calculate_z_level(nd2, frame_number) | |||||
self.assertEqual(result, 0) | |||||
def test_calculate_z_level_2c1f2z(self): | |||||
nd2 = MockNd2Parser(['', 'GFP'], [0], [0, 1]) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 0), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 1), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 2), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 3), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 4), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 5), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 6), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 7), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 8), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 9), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 10), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 11), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 12), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 13), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 14), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 15), 1) | |||||
def test_calculate_z_level_2c3f5z(self): | |||||
nd2 = MockNd2Parser(['', 'GFP'], [0, 1, 2], [0, 1, 2, 3, 4]) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 0), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 1), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 2), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 3), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 4), 2) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 5), 2) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 6), 3) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 7), 3) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 8), 4) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 9), 4) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 10), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 11), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 12), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 13), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 14), 2) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 15), 2) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 16), 3) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 17), 3) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 18), 4) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 19), 4) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 20), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 21), 0) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 22), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 23), 1) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 24), 2) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 25), 2) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 26), 3) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 27), 3) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 28), 4) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 29), 4) | |||||
self.assertEqual(Nd2Parser._calculate_z_level(nd2, 30), 0) | |||||
# from nd2reader.parser import Nd2Parser | |||||
# import unittest | |||||
# | |||||
# | |||||
# class MockNd2Parser(object): | |||||
# def __init__(self, channels, fields_of_view, z_levels): | |||||
# self.channels = channels | |||||
# self.fields_of_view = fields_of_view | |||||
# self.z_levels = z_levels | |||||
# | |||||
# | |||||
# class TestNd2Parser(unittest.TestCase): | |||||
# def test_calculate_field_of_view_simple(self): | |||||
# """ With a single field of view, the field of view should always be the same number (0). """ | |||||
# nd2 = MockNd2Parser([''], [0], [0]) | |||||
# for frame_number in range(1000): | |||||
# result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
# self.assertEqual(result, 0) | |||||
# | |||||
# def test_calculate_field_of_view_two_channels(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP'], [0], [0]) | |||||
# for frame_number in range(1000): | |||||
# result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
# self.assertEqual(result, 0) | |||||
# | |||||
# def test_calculate_field_of_view_three_channels(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP', 'dsRed'], [0], [0]) | |||||
# for frame_number in range(1000): | |||||
# result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
# self.assertEqual(result, 0) | |||||
# | |||||
# def test_calculate_field_of_view_two_fovs(self): | |||||
# nd2 = MockNd2Parser([''], [0, 1], [0]) | |||||
# for frame_number in range(1000): | |||||
# result = Nd2Parser._calculate_field_of_view(nd2, frame_number) | |||||
# self.assertEqual(result, frame_number % 2) | |||||
# | |||||
# def test_calculate_field_of_view_two_fovs_two_zlevels(self): | |||||
# nd2 = MockNd2Parser([''], [0, 1], [0, 1]) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 0), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 1), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 2), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 3), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 4), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 5), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 6), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 7), 1) | |||||
# | |||||
# def test_calculate_field_of_view_two_everything(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP'], [0, 1], [0, 1]) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 0), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 1), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 2), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 3), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 4), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 5), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 6), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, 7), 1) | |||||
# | |||||
# def test_calculate_field_of_view_7c2f2z(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0, 1], [0, 1]) | |||||
# for i in range(14): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
# for i in range(14, 28): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
# for i in range(28, 42): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
# for i in range(42, 56): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
# for i in range(56, 70): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
# for i in range(70, 84): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
# | |||||
# def test_calculate_field_of_view_2c3f5z(self): | |||||
# """ All prime numbers to elucidate any errors that won't show up when numbers are multiples of each other """ | |||||
# nd2 = MockNd2Parser(['', 'GFP'], [0, 1, 2], [0, 1, 2, 3, 4]) | |||||
# for i in range(10): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
# for i in range(10, 20): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
# for i in range(20, 30): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 2) | |||||
# for i in range(30, 40): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 0) | |||||
# for i in range(40, 50): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 1) | |||||
# for i in range(50, 60): | |||||
# self.assertEqual(Nd2Parser._calculate_field_of_view(nd2, i), 2) | |||||
# | |||||
# def test_calculate_channel_simple(self): | |||||
# nd2 = MockNd2Parser(['GFP'], [0], [0]) | |||||
# for i in range(1000): | |||||
# self.assertEqual(Nd2Parser._calculate_channel(nd2, i), 'GFP') | |||||
# | |||||
# def test_calculate_channel(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0], [0]) | |||||
# for i in range(1000): | |||||
# for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7): | |||||
# self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel) | |||||
# | |||||
# def test_calculate_channel_7c2fov1z(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], [0, 1], [0]) | |||||
# for i in range(1000): | |||||
# for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7): | |||||
# self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel) | |||||
# | |||||
# def test_calculate_channel_ludicrous_values(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], list(range(31)), list(range(17))) | |||||
# for i in range(10000): | |||||
# for n, channel in enumerate(['', 'GFP', 'dsRed', 'dTomato', 'lulzBlue', 'jimbotronPurple', 'orange'], start=i*7): | |||||
# self.assertEqual(Nd2Parser._calculate_channel(nd2, n), channel) | |||||
# | |||||
# def test_calculate_z_level(self): | |||||
# nd2 = MockNd2Parser([''], [0], [0]) | |||||
# for frame_number in range(1000): | |||||
# result = Nd2Parser._calculate_z_level(nd2, frame_number) | |||||
# self.assertEqual(result, 0) | |||||
# | |||||
# def test_calculate_z_level_1c1f2z(self): | |||||
# nd2 = MockNd2Parser([''], [0], [0, 1]) | |||||
# for frame_number in range(1000): | |||||
# result = Nd2Parser._calculate_z_level(nd2, frame_number) | |||||
# self.assertEqual(result, frame_number % 2) | |||||
# | |||||
# def test_calculate_z_level_31c17f1z(self): | |||||
# nd2 = MockNd2Parser(list(range(31)), list(range(17)), [0]) | |||||
# for frame_number in range(1000): | |||||
# result = Nd2Parser._calculate_z_level(nd2, frame_number) | |||||
# self.assertEqual(result, 0) | |||||
# | |||||
# def test_calculate_z_level_2c1f2z(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP'], [0], [0, 1]) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 0), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 1), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 2), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 3), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 4), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 5), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 6), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 7), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 8), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 9), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 10), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 11), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 12), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 13), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 14), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 15), 1) | |||||
# | |||||
# def test_calculate_z_level_2c3f5z(self): | |||||
# nd2 = MockNd2Parser(['', 'GFP'], [0, 1, 2], [0, 1, 2, 3, 4]) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 0), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 1), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 2), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 3), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 4), 2) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 5), 2) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 6), 3) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 7), 3) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 8), 4) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 9), 4) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 10), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 11), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 12), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 13), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 14), 2) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 15), 2) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 16), 3) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 17), 3) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 18), 4) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 19), 4) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 20), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 21), 0) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 22), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 23), 1) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 24), 2) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 25), 2) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 26), 3) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 27), 3) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 28), 4) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 29), 4) | |||||
# self.assertEqual(Nd2Parser._calculate_z_level(nd2, 30), 0) |
@ -0,0 +1,7 @@ | |||||
import unittest | |||||
from nd2reader.driver import get_driver | |||||
class TestDriver(unittest.TestCase): | |||||
def test_get_driver(self): | |||||
pass |
@ -0,0 +1,16 @@ | |||||
import unittest | |||||
from nd2reader.driver.version import parse_version | |||||
class VersionTests(unittest.TestCase): | |||||
def test_parse_version_2(self): | |||||
data = 'ND2 FILE SIGNATURE CHUNK NAME01!Ver2.2' | |||||
actual = parse_version(data) | |||||
expected = (2, 2) | |||||
self.assertTupleEqual(actual, expected) | |||||
def test_parse_version_3(self): | |||||
data = 'ND2 FILE SIGNATURE CHUNK NAME01!Ver3.0' | |||||
actual = parse_version(data) | |||||
expected = (3, 0) | |||||
self.assertTupleEqual(actual, expected) |