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@ -37,6 +37,12 @@ class Parser(object): |
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def calculate_image_properties(self, index): |
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def calculate_image_properties(self, index): |
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"""Calculate FOV, channels and z_levels |
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"""Calculate FOV, channels and z_levels |
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Args: |
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index(int): the index (which is simply the order in which the image was acquired) |
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Returns: |
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tuple: tuple of the field of view, the channel and the z level |
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""" |
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""" |
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field_of_view = self._calculate_field_of_view(index) |
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field_of_view = self._calculate_field_of_view(index) |
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channel = self._calculate_channel(index) |
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channel = self._calculate_channel(index) |
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@ -52,6 +58,12 @@ class Parser(object): |
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eliminate this possibility in future releases. For now, you'll need to check if your image is None if you're |
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eliminate this possibility in future releases. For now, you'll need to check if your image is None if you're |
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doing anything out of the ordinary. |
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doing anything out of the ordinary. |
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Args: |
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index(int): the index (which is simply the order in which the image was acquired) |
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Returns: |
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Frame: the image |
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""" |
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""" |
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field_of_view, channel, z_level = self.calculate_image_properties(index) |
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field_of_view, channel, z_level = self.calculate_image_properties(index) |
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channel_offset = index % len(self.metadata["channels"]) |
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channel_offset = index % len(self.metadata["channels"]) |
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@ -66,7 +78,18 @@ class Parser(object): |
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return image |
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return image |
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def get_image_by_attributes(self, frame_number, field_of_view, channel_name, z_level, height, width): |
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def get_image_by_attributes(self, frame_number, field_of_view, channel_name, z_level, height, width): |
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"""Attempts to get Image based on attributes alone. |
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"""Gets an image based on its attributes alone |
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Args: |
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frame_number: the frame number |
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field_of_view: the field of view |
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channel_name: the color channel name |
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z_level: the z level |
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height: the height of the image |
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width: the width of the image |
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Returns: |
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Frame: the requested image |
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""" |
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""" |
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image_group_number = self._calculate_image_group_number(frame_number, field_of_view, z_level) |
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image_group_number = self._calculate_image_group_number(frame_number, field_of_view, z_level) |
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@ -91,6 +114,12 @@ class Parser(object): |
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def _check_version_supported(self): |
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def _check_version_supported(self): |
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"""Checks if the ND2 file version is supported by this reader. |
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"""Checks if the ND2 file version is supported by this reader. |
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Returns: |
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bool: True on supported |
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Raises: |
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InvalidVersionError: Raises an error if the version is unsupported |
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""" |
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""" |
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major_version, minor_version = get_version(self._fh) |
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major_version, minor_version = get_version(self._fh) |
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supported = self.supported_file_versions.get((major_version, minor_version)) or \ |
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supported = self.supported_file_versions.get((major_version, minor_version)) or \ |
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@ -116,6 +145,9 @@ class Parser(object): |
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as some of the bytes contain the value 33, which is the ASCII code for "!". So we iteratively find each label, |
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as some of the bytes contain the value 33, which is the ASCII code for "!". So we iteratively find each label, |
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grab the subsequent data (always 16 bytes long), advance to the next label and repeat. |
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grab the subsequent data (always 16 bytes long), advance to the next label and repeat. |
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Returns: |
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LabelMap: the computed label map |
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""" |
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""" |
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self._fh.seek(-8, 2) |
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self._fh.seek(-8, 2) |
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chunk_map_start_location = struct.unpack("Q", self._fh.read(8))[0] |
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chunk_map_start_location = struct.unpack("Q", self._fh.read(8))[0] |
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@ -126,6 +158,11 @@ class Parser(object): |
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def _calculate_field_of_view(self, index): |
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def _calculate_field_of_view(self, index): |
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"""Determines what field of view was being imaged for a given image. |
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"""Determines what field of view was being imaged for a given image. |
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Args: |
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index(int): the index (which is simply the order in which the image was acquired) |
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Returns: |
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int: the field of view |
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""" |
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""" |
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images_per_cycle = len(self.metadata["z_levels"]) * len(self.metadata["channels"]) |
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images_per_cycle = len(self.metadata["z_levels"]) * len(self.metadata["channels"]) |
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return int((index - (index % images_per_cycle)) / images_per_cycle) % len(self.metadata["fields_of_view"]) |
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return int((index - (index % images_per_cycle)) / images_per_cycle) % len(self.metadata["fields_of_view"]) |
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@ -133,6 +170,12 @@ class Parser(object): |
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def _calculate_channel(self, index): |
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def _calculate_channel(self, index): |
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"""Determines what channel a particular image is. |
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"""Determines what channel a particular image is. |
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Args: |
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index(int): the index (which is simply the order in which the image was acquired) |
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Returns: |
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string: the name of the color channel |
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""" |
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""" |
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return self.metadata["channels"][index % len(self.metadata["channels"])] |
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return self.metadata["channels"][index % len(self.metadata["channels"])] |
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@ -141,6 +184,12 @@ class Parser(object): |
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In the future, this will be replaced with the actual offset in micrometers. |
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In the future, this will be replaced with the actual offset in micrometers. |
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Args: |
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index(int): the index (which is simply the order in which the image was acquired) |
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Returns: |
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The z level |
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""" |
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""" |
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return self.metadata["z_levels"][int( |
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return self.metadata["z_levels"][int( |
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((index - (index % len(self.metadata["channels"]))) / len(self.metadata["channels"])) % len( |
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((index - (index % len(self.metadata["channels"]))) / len(self.metadata["channels"])) % len( |
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@ -150,6 +199,14 @@ class Parser(object): |
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""" |
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""" |
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Images are grouped together if they share the same time index, field of view, and z-level. |
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Images are grouped together if they share the same time index, field of view, and z-level. |
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Args: |
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frame_number: |
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fov: |
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z_level: |
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Returns: |
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int: the image group number |
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""" |
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""" |
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return frame_number * len(self.metadata["fields_of_view"]) * len(self.metadata["z_levels"]) + ( |
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return frame_number * len(self.metadata["fields_of_view"]) * len(self.metadata["z_levels"]) + ( |
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fov * len(self.metadata["z_levels"]) + z_level) |
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fov * len(self.metadata["z_levels"]) + z_level) |
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@ -158,6 +215,13 @@ class Parser(object): |
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""" |
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""" |
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Images are in the same frame if they share the same group number and field of view and are taken sequentially. |
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Images are in the same frame if they share the same group number and field of view and are taken sequentially. |
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Args: |
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image_group_number: |
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field_of_view: |
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z_level: |
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Returns: |
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""" |
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""" |
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return (image_group_number - (field_of_view * len(self.metadata["z_levels"]) + z_level)) / ( |
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return (image_group_number - (field_of_view * len(self.metadata["z_levels"]) + z_level)) / ( |
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len(self.metadata["fields_of_view"]) * len(self.metadata["z_levels"])) |
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len(self.metadata["fields_of_view"]) * len(self.metadata["z_levels"])) |
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@ -168,12 +232,23 @@ class Parser(object): |
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Image data is interleaved for each image set. That is, if there are four images in a set, the first image |
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Image data is interleaved for each image set. That is, if there are four images in a set, the first image |
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will consist of pixels 1, 5, 9, etc, the second will be pixels 2, 6, 10, and so forth. |
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will consist of pixels 1, 5, 9, etc, the second will be pixels 2, 6, 10, and so forth. |
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Returns: |
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dict: the channel offset for each channel |
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""" |
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""" |
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return {channel: n for n, channel in enumerate(self.metadata["channels"])} |
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return {channel: n for n, channel in enumerate(self.metadata["channels"])} |
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def _get_raw_image_data(self, image_group_number, channel_offset, height, width): |
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def _get_raw_image_data(self, image_group_number, channel_offset, height, width): |
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"""Reads the raw bytes and the timestamp of an image. |
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"""Reads the raw bytes and the timestamp of an image. |
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Args: |
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image_group_number: |
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channel_offset: |
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height: |
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width: |
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Returns: |
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""" |
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""" |
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chunk = self._label_map.get_image_data_location(image_group_number) |
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chunk = self._label_map.get_image_data_location(image_group_number) |
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data = read_chunk(self._fh, chunk) |
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data = read_chunk(self._fh, chunk) |
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