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  1. """
  2. Legacy class for backwards compatibility
  3. """
  4. import warnings
  5. from nd2reader import ND2Reader
  6. class Nd2(object):
  7. """ Warning: this module is deprecated and only maintained for backwards compatibility with the non-PIMS version of
  8. nd2reader.
  9. """
  10. def __init__(self, filename):
  11. warnings.warn(
  12. "The 'Nd2' class is deprecated, please consider using the new ND2Reader interface which uses pims.",
  13. DeprecationWarning)
  14. self.reader = ND2Reader(filename)
  15. def __repr__(self):
  16. return "\n".join(["<Deprecated ND2 %s>" % self.reader.filename,
  17. "Created: %s" % (self.date if self.date is not None else "Unknown"),
  18. "Image size: %sx%s (HxW)" % (self.height, self.width),
  19. "Frames: %s" % len(self.frames),
  20. "Channels: %s" % ", ".join(["%s" % str(channel) for channel in self.channels]),
  21. "Fields of View: %s" % len(self.fields_of_view),
  22. "Z-Levels: %s" % len(self.z_levels)
  23. ])
  24. def __enter__(self):
  25. return self
  26. def __exit__(self, exc_type, exc_val, exc_tb):
  27. if self.reader is not None:
  28. self.reader.close()
  29. def __len__(self):
  30. return len(self.reader)
  31. def __getitem__(self, item):
  32. return self.reader[item]
  33. def select(self, fields_of_view=None, channels=None, z_levels=None, start=0, stop=None):
  34. """Select images based on criteria.
  35. Args:
  36. fields_of_view: the fields of view
  37. channels: the color channels
  38. z_levels: the z levels
  39. start: the starting frame
  40. stop: the last frame
  41. Returns:
  42. ND2Reader: Sliced ND2Reader which contains the frames
  43. """
  44. if stop is None:
  45. stop = len(self.frames)
  46. return self.reader[start:stop]
  47. def get_image(self, frame_number, field_of_view, channel_name, z_level):
  48. """Deprecated. Returns the specified image from the ND2Reader class.
  49. Args:
  50. frame_number: the frame number
  51. field_of_view: the field of view number
  52. channel_name: the name of the color channel
  53. z_level: the z level number
  54. Returns:
  55. Frame: the specified image
  56. """
  57. return self.reader.parser.get_image_by_attributes(frame_number, field_of_view, channel_name, z_level,
  58. self.height, self.width)
  59. def close(self):
  60. """Closes the ND2Reader
  61. """
  62. if self.reader is not None:
  63. self.reader.close()
  64. @property
  65. def height(self):
  66. """Deprecated. Fetches the height of the image.
  67. Returns:
  68. int: the pixel height of the image
  69. """
  70. return self.reader.metadata["height"]
  71. @property
  72. def width(self):
  73. """Deprecated. Fetches the width of the image.
  74. Returns:
  75. int: the pixel width of the image
  76. """
  77. return self.reader.metadata["width"]
  78. @property
  79. def z_levels(self):
  80. """Deprecated. Fetches the available z levels.
  81. Returns:
  82. list: z levels.
  83. """
  84. return self.reader.metadata["z_levels"]
  85. @property
  86. def fields_of_view(self):
  87. """Deprecated. Fetches the fields of view.
  88. Returns:
  89. list: fields of view.
  90. """
  91. return self.reader.metadata["fields_of_view"]
  92. @property
  93. def channels(self):
  94. """Deprecated. Fetches all color channels.
  95. Returns:
  96. list: the color channels.
  97. """
  98. return self.reader.metadata["channels"]
  99. @property
  100. def frames(self):
  101. """Deprecated. Fetches all frames.
  102. Returns:
  103. list: list of frames
  104. """
  105. return self.reader.metadata["frames"]
  106. @property
  107. def date(self):
  108. """Deprecated. Fetches the acquisition date.
  109. Returns:
  110. string: the date
  111. """
  112. return self.reader.metadata["date"]
  113. @property
  114. def pixel_microns(self):
  115. """Deprecated. Fetches the amount of microns per pixel.
  116. Returns:
  117. float: microns per pixel
  118. """
  119. return self.reader.metadata["pixel_microns"]