|
|
- # -*- coding: utf-8 -*-
-
- import numpy as np
- import warnings
-
-
- class Image(np.ndarray):
- def __new__(cls, array):
- return np.asarray(array).view(cls)
-
- def __init__(self, array):
- self._timestamp = None
- self._frame_number = None
- self._field_of_view = None
- self._channel = None
- self._z_level = None
-
- def add_params(self, timestamp, frame_number, field_of_view, channel, z_level):
- """
- A wrapper around the raw pixel data of an image.
-
- :param timestamp: The frame number relative to the .
- :type timestamp: int
- :param timestamp: The number of milliseconds after the beginning of the acquisition that this image was taken.
- :type timestamp: int
- :param field_of_view: The label for the place in the XY-plane where this image was taken.
- :type field_of_view: int
- :param channel: The name of the color of this image
- :type channel: str
- :param z_level: The label for the location in the Z-plane where this image was taken.
- :type z_level: int
-
- """
- self._timestamp = timestamp
- self._frame_number = int(frame_number)
- self._field_of_view = field_of_view
- self._channel = channel
- self._z_level = z_level
-
- @property
- def height(self):
- return self.shape[1]
-
- @property
- def width(self):
- return self.shape[0]
-
- @property
- def field_of_view(self):
- """
- Which of the fixed locations this image was taken at.
-
- :rtype int:
-
- """
- return self._field_of_view
-
- @property
- def timestamp(self):
- """
- The number of seconds after the beginning of the acquisition that the image was taken. Note that for a given
- field of view and z-level offset, if you have images of multiple channels, they will all be given the same
- timestamp. No, this doesn't make much sense. But that's how ND2s are structured, so if your experiment depends
- on millisecond accuracy, you need to find an alternative imaging system.
-
- :rtype float:
-
- """
- return self._timestamp / 1000.0
-
- @property
- def frame_number(self):
- return self._frame_number
-
- @property
- def channel(self):
- """
- The name of the filter used to acquire this image. These are user-supplied in NIS Elements.
-
- :rtype str:
-
- """
- return self._channel
-
- @property
- def z_level(self):
- """
- The vertical offset of the image. These are simple integers starting from 0, where the 0 is the lowest
- z-level and each subsequent level incremented by 1.
-
- For example, if you acquired images at -3 µm, 0 µm, and +3 µm, your z-levels would be:
-
- -3 µm: 0
- 0 µm: 1
- +3 µm: 2
-
- :rtype int:
-
- """
- return self._z_level
-
- @property
- def data(self):
- warnings.warn("Image objects now directly subclass Numpy arrays, so using the data attribute will be removed in the near future.", DeprecationWarning)
- return self
|