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10 years ago
10 years ago
10 years ago
  1. import logging
  2. from nd2reader.service import BaseNd2
  3. from nd2reader.model import Image, ImageSet
  4. log = logging.getLogger("nd2reader")
  5. log.addHandler(logging.StreamHandler())
  6. log.setLevel(logging.DEBUG)
  7. class Nd2(BaseNd2):
  8. def __init__(self, filename):
  9. super(Nd2, self).__init__(filename)
  10. def get_image(self, timepoint, fov, channel_name, z_level):
  11. image_set_number = self._calculate_image_set_number(timepoint, fov, z_level)
  12. timestamp, raw_image_data = self._reader.get_raw_image_data(image_set_number, self.channel_offset[channel_name])
  13. return Image(timestamp, raw_image_data, fov, channel_name, z_level, self.height, self.width)
  14. def __iter__(self):
  15. """
  16. Just return every image in order (might not be exactly the order that the images were physically taken, but it will
  17. be within a few seconds). A better explanation is probably needed here.
  18. """
  19. for i in range(self._image_count):
  20. for fov in range(self._field_of_view_count):
  21. for z_level in range(self._z_level_count):
  22. for channel in self.channels:
  23. image = self.get_image(i, fov, channel.name, z_level)
  24. if image.is_valid:
  25. yield image
  26. def image_sets(self, field_of_view, timepoints=None, channels=None, z_levels=None):
  27. """
  28. Gets all the images for a given field of view and
  29. """
  30. timepoint_set = xrange(self._timepoint_count) if timepoints is None else timepoints
  31. channel_set = [channel.name for channel in self.channels] if channels is None else channels
  32. z_level_set = xrange(self._z_level_count) if z_levels is None else z_levels
  33. for timepoint in timepoint_set:
  34. image_set = ImageSet()
  35. for channel_name in channel_set:
  36. for z_level in z_level_set:
  37. image = self.get_image(timepoint, field_of_view, channel_name, z_level)
  38. if image.is_valid:
  39. image_set.add(image)
  40. yield image_set