From a5aacf22940b79de53fb802fba16b23fffd0b799 Mon Sep 17 00:00:00 2001 From: "lorenzo.zolfanelli93" Date: Thu, 27 Aug 2020 15:08:19 +0000 Subject: [PATCH] Update on Overleaf. --- chapters/3-methods.tex | 56 +- images/tension.pdf | Bin 0 -> 13925 bytes images/three_beads_fbON_bound.pdf | Bin 0 -> 26503 bytes images/three_beads_fbON_bound.svg | 1889 +++++++++++++++++++++++++++ images/three_beads_fbON_unbound.pdf | Bin 0 -> 25227 bytes images/three_beads_fbON_unbound.svg | 1837 ++++++++++++++++++++++++++ images/three_beads_tension.pdf | Bin 0 -> 24875 bytes images/three_beads_tension.svg | 1678 ++++++++++++++++++++++++ images_src/tension.svg | 1494 +++++++++++++++++++++ main.tex | 1 + 10 files changed, 6952 insertions(+), 3 deletions(-) create mode 100644 images/tension.pdf create mode 100644 images/three_beads_fbON_bound.pdf create mode 100644 images/three_beads_fbON_bound.svg create mode 100644 images/three_beads_fbON_unbound.pdf create mode 100644 images/three_beads_fbON_unbound.svg create mode 100644 images/three_beads_tension.pdf create mode 100644 images/three_beads_tension.svg create mode 100644 images_src/tension.svg diff --git a/chapters/3-methods.tex b/chapters/3-methods.tex index dda3503..8b6e366 100644 --- a/chapters/3-methods.tex +++ b/chapters/3-methods.tex @@ -352,7 +352,7 @@ i valori di $\beta$ e $k$ imponendo i valori noti riportati in tabella \ref{tab: In figura \ref{fig:psd} si riporta una distribuzione spettrale tipica confrontata con la funzione teorica. -\begin{figure}[ht] +\begin{figure}[htpb] \centering \includegraphics{images/PSD.pdf} \caption[scale=0.7]{Densità spettrale di rumore per la posizione di una trappola @@ -365,7 +365,7 @@ trappola è possibile interpolare i valori per ogni possibile posizione intermeda. Per fare questo si usano delle funzioni polinomiali di ordine 3, come mostrato in figura \ref{fig:trap_ccurves}. -\begin{figure}[ht] +\begin{figure}[htpb] \centering \includegraphics[scale=0.8]{images/calibration_curves.pdf} \caption{Andamento e interpolazione dei valori di $k$ e $\beta$.} @@ -407,6 +407,13 @@ verificarsi due situazioni: sarà applicata una tensione pari a quella selezionata. \end{itemize} +\begin{figure}[ht] + \centering + \includegraphics{images/tension.pdf} + \caption{\textit{Force-clamp} con una trappola.} + \label{fig:tension} +\end{figure} + Osservando i tracciati temporali della posizione relativa della microsfera è possibile individuare la transizione tra questi due regimi, sia attraverso la velocità di variazione della posizione, @@ -417,9 +424,52 @@ per diversi valori di tensione selezionati è possibile caratterizzare quantitativamente la dipendenza dalle sollecitazioni esterne del legame analizzato. - +Per realizzare sperimentalmetne questa misura + \section{Saggio a tre sfere} \label{sec:three-beads} + +Visto che nelle giunzioni aderenti la trasmissione degli sforzi +meccanici è spesso media tra proteine filamentose (come la +\textit{F-actina}) risulta particolarmente utile sviluppare un +saggio in cui, usando due trappole, è possibile mettere in tensione +una proteina filamentosa, simulando ad esempio lo stato in cui si +può trovare la \textit{F-actina} nel citoscheletro. + +\begin{figure}[h] +\centering + \begin{subfigure}{0.45\linewidth} + \centering + \includegraphics[width=\linewidth]{images/three_beads_tension.pdf} + \caption{} + \label{fig:feedback-off} +\end{subfigure} +\\[\baselineskip] +\begin{subfigure}{0.45\linewidth} + \centering + \includegraphics[width=\linewidth]{images/three_beads_fbON_unbound.pdf} + \caption{} + \label{fig:feedback-on-unbound} +\end{subfigure} +\hfill +\begin{subfigure}[H]{0.45\linewidth} + \centering + \includegraphics[width=\linewidth]{images/three_beads_fbON_bound.pdf} + \caption{} + \label{fig:feedback-on-bound} +\end{subfigure} +\centering +\caption{(a) Ciclo di retroazione disattivato, microsfere in posizione di equilibrio. La tensione del filamento può essere aggiustata modificando la distanza delle trappole. (b) Ciclo di retroazione +attivato, nessuna molecola immobilizzata legata al filamento. Le +trappole si muovono indefinitamente per ``inseguire'' la posizione +corrispondente alla forza richiesta. (c) Il filamento si lega con +una molecola immobilizzata sul vetrino, la microsfera raggiunge la +posizione target e lo spostamento delle trappole si arresta. La forza +applicata sul legame è pari a quella selezionata}. +\label{fig:three_beads} +\end{figure} + +In figura \ref{fig:three_beads} è rappresentato lo schema realizzato \section{Fluorescenza di singola molecole} \label{sec:single_molecule_fluorescence} diff --git a/images/tension.pdf b/images/tension.pdf new file mode 100644 index 0000000000000000000000000000000000000000..6ebb4e82a59366142e4ae1b7f76a2e07eba4c9af GIT binary patch literal 13925 zcmdse2_TeR+kd4}LM5_f8HJE#_5~%zl3j>4F&K<6m{E2SNs4yaWnW6kp0rpiSqdp@ zmPGb!#Z!IvJ!35C`QGP!-v9r5-|w4dnz^sDUFSO2`JLZ&&LOI=q9zTKL9vMD4QI`< zAR$l)*}{%RK>;GGO?0%O+Cor($bba`fyktNV zp1Xz8cUx+;EUJ9mvkiqSXOzaY3^oN?e$=gMm=^3jimy>F2^3S_ZNMfiv61^Thk=8L zfwFz0VSQ-uE#(WE>XQ5}EzD?-B!9gzsjK)#<>YhxiA5}Xne6m=EK>d32TW!uAxef> zMILA5&)+t=W4|H7@_dArGk(G z=1Rk?{>XirTsU*{)uo%**VB)xqo;Y`B=$EU72LdMxr1gpv0M>JEV9PVjd`keH4Xb- zmv6XjoL91!L*#kWn#e0Wat00ct))BiSaBbTa<8dgZLpo)`PwJ(cZfJaTg1-F#}tnW zthVefL6!X%b$Alh%GoV6l{D{&`dtuYDlpogc5;`DShu;wflr@CN!kwlS3Ph0LZ_H~ 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+1,1678 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + image/svg+xml + + + + + + + + + + + + + + + + + + + + + + Δx + + + + + + + + + + Δx + + filamentoin tensione + |F0| = kΔx + + diff --git a/images_src/tension.svg b/images_src/tension.svg new file mode 100644 index 0000000..04c1817 --- /dev/null +++ b/images_src/tension.svg @@ -0,0 +1,1494 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + image/svg+xml + + + + + + + + + + + + + + + + + + + + + + Δx + + diff --git a/main.tex b/main.tex index 7cba88c..a8b1172 100644 --- a/main.tex +++ b/main.tex @@ -23,6 +23,7 @@ \usepackage{caption} \captionsetup[figure]{font=small,labelfont=bf} \captionsetup[table]{font=small,labelfont=bf} +\usepackage{subcaption} % ===== MATHS