From 8be013d7f78ed6c654e7163735fad5b8aef945e6 Mon Sep 17 00:00:00 2001 From: "lorenzo.zolfanelli93" Date: Thu, 27 Aug 2020 22:48:04 +0000 Subject: [PATCH] Update on Overleaf. --- chapters/2-setup.tex | 4 +- chapters/3-methods.tex | 38 +- images/forceclamp_feedback.pdf | Bin 0 -> 40074 bytes {images => images_src}/flow_cell.svg | 0 images_src/forceclamp_feedback.svg | 4134 ++++++++++++++++++++++++++ 5 files changed, 4166 insertions(+), 10 deletions(-) create mode 100644 images/forceclamp_feedback.pdf rename {images => images_src}/flow_cell.svg (100%) create mode 100644 images_src/forceclamp_feedback.svg diff --git a/chapters/2-setup.tex b/chapters/2-setup.tex index 1384818..deb81d3 100644 --- a/chapters/2-setup.tex +++ b/chapters/2-setup.tex @@ -391,7 +391,7 @@ due telescopi realizzati con lenti piano-convesse. L'emissione dei laser a diodo deve essere filtrata attraverso filtri passa-banda stretti per evitare che radiazione a lunghezze d'onda spurie diffonda nel campione e contamini l'emissione di -fluroescenza. +fluorescenza. I tre fasci laser vengono combinati in un unico percorso ottico e ingranditi di un fattore 10x per mezzo di un ulteriore telescopio @@ -408,7 +408,7 @@ trasmettere tre finestre di emissione nel quale ricadono i fluorofori più comuni. La fluorescenza riemessa all'indietro dal campione, e quindi trasmessa dal dicroico di fluorescenza, è combinata con l'illuminazione a luce -trasmessa, da cui sarà disaccopiata per mezzo di un successivo +trasmessa, da cui sarà disaccoppiata per mezzo di un successivo dicroico. Il percorso di raccolta dell'emissione di fluorescenza, infatti, diff --git a/chapters/3-methods.tex b/chapters/3-methods.tex index 7bdd134..8e46847 100644 --- a/chapters/3-methods.tex +++ b/chapters/3-methods.tex @@ -442,6 +442,17 @@ progettato in ambiente LabVIEW, e comunica con un apposito programma in esecuzione sul PC di controllo dell'esperimento per configurare i parametri sperimentali e memorizzare i tracciati (\texttt{Force-Clamp Control}). +In figura \ref{fig:forceclamp-feedback} è mostrato una schema +semplificato del ciclo di retroazione implementato per ciascuna +trappola. + +\begin{figure}[ht] + \centering + \includegraphics{images/forceclamp_feedback.pdf} + \caption{Caption} + \label{fig:forceclamp-feedback} +\end{figure} + \section{Saggio a tre sfere} \label{sec:three-beads} @@ -527,19 +538,30 @@ finestre riportate in tabella \ref{tab:fluo_lambda}: \begin{table}[ht] \centering - \begin{tabular}{c c} + \begin{tabular}{c c c} \toprule - $\lambda$ eccitazione & $\lambda$ emissione \\ + $\lambda$ eccitazione [\si{nm}] & $\lambda$ emissione [\si{nm}] & Potenza massima [\si{mW}] \\ \midrule - \SI{488}{\nm} & \SIrange{502.5}{518.5}{\nm} \\ - \SI{532}{\nm} & \SIrange{550}{613}{\nm} \\ - \SI{635}{\nm} & \SIrange{663}{700}{\nm} \\ + \num{488} & \numrange{502.5}{518.5} & \num{1.6}\\ + \num{532} & \numrange{550.0}{613.0} & \num{1.1}\\ + \num{635} & \numrange{663.0}{700.0} & \num{2.4} \\ \bottomrule \end{tabular} - \caption{Lunghezze d'onda di eccitazione e emissione compatibili con l'apparato sperimentale.} + \caption{Lunghezze d'onda di eccitazione e emissione compatibili con l'apparato sperimentale, e potenza + elettromagnetica toatale immessa nel campo visivo.} \label{tab:fluo_lambda} \end{table} - +Per ciascuna lunghezza d'onda è possibile ottenere una potenza totale nel campo +visivo raccolto dal sensore EMCCD $\SI{1}{mW}$. +Per confrontare le proprietà di fluorofori a diverse lunghezze d'onda scegliamo +di impostare l'emissione di tutte le sorgenti laser in modo da avere sempre +$\SI{1}{\mW}$ di potenza ottica sul campione. +Per rendere uniforme la distribuzione di potenza nel campo visivo si tronca il +fascio gaussiano prima di focalizzarlo sull'obiettivo a un raggio pari circa +a 1/15 di quello iniziale ( + \section{TIRF e illuminazione a modi di galleria} - \label{sec:gallery_mode} \ No newline at end of file + \label{sec:gallery_mode} + + \ No newline at end of file diff --git a/images/forceclamp_feedback.pdf b/images/forceclamp_feedback.pdf new file mode 100644 index 0000000000000000000000000000000000000000..34593b4cec8da6499bdba4f219c6b598dcf331af GIT binary patch literal 40074 zcmeFa2Ow5&`#4^9i4aN1BO@c@@eI!sDLY$;>^-wrw(MC(6he_mMp=pMy@j%~_Xvq( z{?GHEkH%;9`@G-p`~JT#hjY$-&bjaFzOU=P_PGy(tdIyBm>qVMA#Xl&<0urw39`^J zIm*im;*iugH?%bZ!GI#UqaYB7L&VtBR^J-fn`+zY3+d}x=;FgMp%Y)}2?jAck2-oF#Q$@(mBC%eb=-1(;C8nd7CLWe)J>DsZl4@QaQJT~VFs?cNATVe7L*$rE{9|Ps80|(uC(Oqv z>$s(gjOq5L`B`YG>qS>sw40GZ2BY68OCyU>mXjCAdJ+hsF}tLMx) 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