From 478908bc478f3a97545d37781f12324b289910ff Mon Sep 17 00:00:00 2001 From: "lorenzo.zolfanelli93" Date: Sat, 15 Aug 2020 19:15:04 +0000 Subject: [PATCH] Update on Overleaf. --- chapters/1-introduction.tex | 30 +- images/hilo.pdf | Bin 0 -> 12999 bytes images_src/hilo.svg | 2758 +++++++++++++++++++++++++++++++++++ main.tex | 2 + 4 files changed, 2789 insertions(+), 1 deletion(-) create mode 100644 images/hilo.pdf create mode 100644 images_src/hilo.svg diff --git a/chapters/1-introduction.tex b/chapters/1-introduction.tex index f4595a4..29951e8 100644 --- a/chapters/1-introduction.tex +++ b/chapters/1-introduction.tex @@ -355,7 +355,7 @@ irreversibile (vedi figura \ref{fig:biotin-streptavidin}). \begin{figure}[ht] \centering \includegraphics[width=0.5\linewidth]{images/biotin-streptavidin.pdf} - \caption{Manipolazione di una proteina target utilizzando una + \caption{Manipolazione di una proteina bersaglio utilizzando una microsfera intrappolata e il legame biotina-streptavidina.} \label{fig:biotin-streptavidin} \end{figure} @@ -811,4 +811,32 @@ in microscopia di fluorescenza, $\lambda_0 = \SI{532}{\nm}$, e un angolo di incidenza $\theta_c \approx \SI{62}{\degree}$, otteniamo una profondità di penetrazione $d_p$ di circa \SI{300}{\nm}. +Questi numeri ci danno un'idea del limiti di applicazione del campo +di applicazione della microscopia TIRF. Quando è necessario +individuare fluorofori che si trovano a una profondità maggiore +di poche centinaia di nanometri rispetto al vetrino coprioggetti +questa tecnica non è più utilizzabile. + +Per ottimizzare il rapporto segnale/rumore quando si deve lavorare +a profondità maggiori è stata sviluppata un'altra tecnica, la HILO, +che sfrutta le proprietà della rifrazione per comprimere lo spessore +di campione illuminato. + \subsection{HILO} + +La microscopia a fogli di luce altamente inclinati +(\textit{Highly Inclined Laminated Optical Sheet microscopy}, HILO) +permette, analogamente alla TIRF, di illuminare uno spessore +ridotto del campione. + + +\begin{figure}[ht] + \centering + \includegraphics[width=\linewidth]{images/hilo.pdf} + \caption{Schema di illuminazione HILO. Effetto + della rifrazione sulla dimensione del fascio (a sinistra) e + relazione tra quota del campione illuminata e spostamento + orizzontale del fascio (a destra).} + \label{fig:hilo} +\end{figure} + diff --git a/images/hilo.pdf b/images/hilo.pdf new file mode 100644 index 0000000000000000000000000000000000000000..9062ac50b53e86afe41beee94c1175d3d7cb6733 GIT binary patch literal 12999 zcmeHuXHZm2*RFs>$r&UJIm65lhO9)%AUVST1B~QhKqM+TCy9bckgNg{BxjH;K>;O5 zPKx9lyaRgPbKdiQ_pAEq-dlBloL#d+ukPKe*VAk7TD_hdJhtl1H)nW4 z03g7{>H(glBtSq7=4^+w2MD7?+IRo}KtK`hh=d_fXGbU!CJ(cAv4P=9OXImC5iqC| zo>ykINq;O`o!iVF4}vcnk?4Z>7IOmwi`NJ4#q{zMH?>}%t>WW=uWlMQ8Q=ZbbTcWp z14{X6uvxTO0-33o<5VyG_IB?zo_=;9oUgqz^Wn$pVqw7L;@8W=LW@hpbPL0si)y-- z*#I&B(?yT-6N6MgiL%K!!y(^J-J$rg`4Jfj|C6zu!HYICyCz?O(Y-MX|C7Bnjsq|D zud!O~MpjbNai(ts!@RxLh0NxUMf*IS8{pcg?6&vNeehgn`RGr1@-s|g)&hC3`S8cW z?&b0k*s~uzk33Ix{mJ?BqVVip0M()RWk>$vi`W-LA2bg%Nm?iF-+6IQ%HQ(v$MM7> z(U0OI({mb~r(3%;!{y^hyrIo~)7*LE@H$;X 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