`nd2reader` is a pure-Python package that reads images produced by NIS Elements 4.0+. It has only been definitively tested on NIS Elements 4.30.02 Build 1053. Support for older versions is planned.
`nd2reader` is a pure-Python package that reads images produced by NIS Elements 4.0+. It has only been definitively tested on NIS Elements 4.30.02 Build 1053. Support for older versions is being actively worked on.
.nd2 files contain images and metadata, which can be split along multiple dimensions: time, fields of view (xy-plane), focus (z-plane), and filter channel.
.nd2 files contain images and metadata, which can be split along multiple dimensions: time, fields of view (xy-plane), focus (z-plane), and filter channel.
@ -10,11 +10,13 @@
### Installation
### Installation
If you don't already have the packages `numpy` and `six`, they will be installed automatically:
`pip3 install nd2reader` for Python 3.x
`pip3 install nd2reader` for Python 3.x
`pip install nd2reader` for Python 2.x
`pip install nd2reader` for Python 2.x
If you don't already have the packages `numpy` and `six`, they will be installed automatically.
`nd2reader` is an order of magnitude faster in Python 3. I recommend using it unless you have no other choice.
### ND2s
### ND2s
@ -27,38 +29,25 @@ A quick summary of ND2 metadata can be obtained as shown below.
Created: 2014-11-11 15:59:19
Created: 2014-11-11 15:59:19
Image size: 1280x800 (HxW)
Image size: 1280x800 (HxW)
Image cycles: 636
Image cycles: 636
Channels: '', 'GFP'
Channels: 'brightfield', 'GFP'
Fields of View: 8
Fields of View: 8
Z-Levels: 3
Z-Levels: 3
```
```
You can also get some metadata about the nd2 programatically:
You can iterate over each image in the order they were acquired:
```python
```python
>>> nd2.height
1280
>>> nd2.width
800
>>> len(nd2)
30528
```
`Nd2` is also a context manager, if you care about that sort of thing:
```
>>> import nd2reader
>>> with nd2reader.Nd2("/path/to/my_images.nd2") as nd2:
... for image in nd2:
... do_something(image)
import nd2reader
nd2 = nd2reader.Nd2("/path/to/my_images.nd2")
for image in nd2:
do_something(image)
```
```
### Images
`Image` objects are just Numpy arrays with some extra metadata bolted on:
`Image` objects are just Numpy arrays with some extra metadata bolted on: